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GE17-5280-01

nProtein A Sepharose™ 4 Fast Flow

Cytiva 17-5280-01, pack of 5 mL

同義詞:

Sepharose 4 Fast Flow

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56
暫時無法取得訂價和供貨情況

ligand

native protein A (S. aureus)

包裝

pack of 5 mL

製造商／商標名

Cytiva 17-5280-01

儲存條件

(20% Ehtanol)

基質

4% cross-linked agarose

平均直徑

90 μm (d50v)

cleaning

2-10

工作範圍

3-9

容量

>30 mg binding capacity(human IgG/ml)

適合性

suitable for bioprocess medium

儲存溫度

2-8°C

相關類別

一般說明

nProtein A Sepharose™ 4 Fast Flow is native protein A coupled to Sepharose™ 4 Fast Flow for the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.

nProtein A Sepharose™ 4 Fast Flow is native protein A coupled to the well established Sepharose™ 4 Fast Flow base matrix. The native protein A ligand is produced by fermenting a selected strain of Staphylococcus aureus. The purified protein is coupled to the cross-linked 4% agarose base matrix by the cyanogen bromide technique, giving a highly stable medium with minimal non-specific adsorption. nProtein A Sepharose™ 4 Fast Flow is manufactured without using animal-derived components.

nProtein A Sepharose™ 4 Fast Flow has nearly twice the total IgG binding capacity of Protein A Sepharose™ CL-4B, and is suitable for recovery and purification of monoclonal antibodies from cell culture at both laboratory and process scale. nProtein A Sepharose™ 4 Fast Flow was developed and tested in co-operation with leading manufacturers of purified monoclonal antibody products, and is used in routine commercial production.

As member of the BioProcess media range, nProtein A Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特點和優勢

  • Replaces Protein A Sepharose™ 4 Fast Flow, the first Cytiva Protein A medium for large-scale purification of antibodies.
  • Used in routine commercial production of monoclonal antibodies
  • Free from animal-derived components.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

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分析證明 (COA)

Lot/Batch Number

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文章

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

條款

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

牽引測試

本頁提供不同的下拉分析法資訊，以進一步分離多蛋白複合物，鑑定其成分與 Cytiva 的產品。

Protein A-Based Separation with Cytiva Products

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

相關內容

Performing a Separation with GE Healthcare Products Based on Protein A

Protein A is derived from a strain of Staphylococcus aureus and contains five regions that bind to the Fc region of IgG. As an affinity ligand, protein A is coupled to Sepharose so that these regions are free to bind IgG.

蛋白質下拉技術

利用親和、GST pull-down、TAP 和共免疫沉淀等方法，使用下拉試驗研究體外蛋白質與蛋白質之間的相互作用。

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

蛋白質下拉技術

利用親和、GST pull-down、TAP 和共免疫沉淀等方法，使用下拉試驗研究體外蛋白質與蛋白質之間的相互作用。

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