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重要文件

GE17-5068-01

Sigma-Aldrich

SOURCE 15RPC ST 4.6/100

pkg of 1 column

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About This Item

分類程式碼代碼:
41115700
NACRES:
SB.52
暫時無法取得訂價和供貨情況

ligand

matrix surface

材料

polypropylene column

特點

autoclavable 20 min at 121°C

包裝

pkg of 1 column

參數

flow rate
0.5 ml/min - 2.5 ml/min flow rate
580 psi

技術

HPLC: suitable

床尺寸

4.6 mm × 100 mm

床體積

1.7 mL

柱內徑

4.6 mm

基質

polymeric
polystyrene/divinylbenzene

基質活性組

polystyrene-divinylbenzene phase

樣品平均尺寸

15 μm

cleaning

1-14

工作範圍

1-12

容量

~10 mg binding capacity

適合性

suitable for bioprocess medium

分離技術

reversed phase

一般說明

SOURCE 15RPC ST 4.6/100 is a prepacked stainless steel column which provides fast, high-capacity preparative protein purification with reversed phase chromatography.
~10 mg BSA /ml medium at 300 cm/h, ~30 mg bacitracin/ml medium at 300 cm/h, ~50 mg insulin/ml medium at 300 cm/h

應用

SOURCE 15RPC is based on rigid, monosized 15 μm diameter polystyrene/divinyl benzene beads. The matrix has outstanding selectivity for RPC. The monodispersity of the beads yields stable beds, Low back pressures and excellent results at high flow rates.With its high physical and chemical stability and very high batch-to-batch reproducibility, SOURCE 15RPC is well suited for all stages of an industrial scale operation - from research and process development through scale-up and into production. SOURCE 15RPC offers properties superior to those of other polymeric matrices.

特點和優勢

  • Wide pH range (1-12), outstanding selectivity, chemical resistance, high capacity, and high resolution at high fLow rates.
  • Excellent scalability, from RESOURCE to FineLINE columns.
  • using ÄKTA® design and other high-performance liquid chromatography systems.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol or 70% Acetonitrile)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

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法律資訊

RESOURCE is a trademark of Cytiva
SOURCE is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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分析證明 (COA)

Lot/Batch Number

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文章

由於洗脫液品質差,色譜圖中出現 「鬼影」;痕量有機雜質濃縮,造成未知峰。

“Ghosting” in chromatograms due to poor eluent quality; trace organic impurities concentrate, causing unknown peaks.

The different stages of working with SOURCE™ (Cytiva) for high-resolution separation are discussed, from purification to polishing and scaling up

This page discusses column packing and preparation techniques for reverse phase chromatography.

條款

In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.

相關內容

This page discusses column packing and preparation techniques for reverse phase chromatography

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