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GE17-5053-01

HiTrap® Q Fast Flow

Cytiva 17-5053-01, pack of 5 × 1 mL

同義詞:

Ion exchange chromatography column

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

ligand

quaternary amine

描述

Ion Exchanger Type (value)

包裝

pack of 5 × 1 mL

製造商／商標名

Cytiva 17-5053-01

參數

<4 mL/min flow rate
42 psi

床尺寸

7 mm × 25 mm

床體積

1 mL

柱內徑

7 mm

基質

6% cross-linked agarose

粒徑

45-165 μm

平均直徑

90 μm

cleaning

2-14

工作範圍

2-12

適合性

suitable for bioprocess medium

一般說明

HiTrap® Q FF is prepacked with Q Sepharose™ Fast Flow, and is a strong anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.

Q, SP, DEAE, and CM Sepharose™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.

特點和優勢

  • Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
  • The industry standard for ion exchange chromatography.
  • High flow rates and good scale-up potential.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
  • Predictable scale-up

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

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分析證明 (COA)

Lot/Batch Number

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文章

Purifcation or Removal of Serine Proteases, e.g. Thrombin and Trypsin, and Zymogens

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.

純化或去除絲氨酸蛋白酶，例如凝血酶和胰蛋白酶，以及酵母菌

本頁說明如何使用 Cytiva 的 HiTrap Benzamidine FF 和 Benzamidine Sepharose 4 Fast Flow 去除重組產品中的絲氨酸蛋白酶，例如凝血酶和胰蛋白酶，以及zymogens。

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Performing a Purification with Calmodulin Sepharose 4B

This page shows how to purify ATPases, adenylate cyclases, protein kinases, phosphodiesterases and neurotransmitters by affinity chromatography using Calmodulin Sepharose 4B from Cytiva.

Refolding: Ion Exchange Chromatography

Ion exchange chromatography and Superdex column analysis facilitate protein refolding with step-by-step guidance.

重新折疊：離子交換色譜法

離子交換色譜法和 Superdex 色譜柱分析可在逐步指導下促進蛋白質重折疊。

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from Cytiva.

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相關內容

Performing a Purification with Calmodulin Sepharose 4B

This page shows how to purify ATPases, adenylate cyclases, protein kinases, phosphodiesterases and neurotransmitters by affinity chromatography using Calmodulin Sepharose 4B from GE Healthcare.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

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