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GE17-1167-01

Sigma-Aldrich

HiPrep™ 16/60 Sephacryl® S-300 HR

Cytiva 17-1167-01, column L × I.D. 60 cm × 16 mm, 50 μm avg. part. size

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About This Item

分類程式碼代碼:
41115700
NACRES:
SB.52
暫時無法取得訂價和供貨情況

材料

polypropylene

分子量

10000-1500000 Da (globular proteins)

包裝

pkg of 1 ea

製造商／商標名

Cytiva 17-1167-01

參數

<1 mL/min flow rate
<5 mL sample volume
0.15 Mpa (22 psi)

床體積

120 mL

柱長度 × 內徑

60 cm × 16 mm

基質

dextran-acrylamide particle platform
allyl dextran and N, N’-methylenebisacrylamide
polymeric

基質活性組

dextran-acrylamide phase

樣品平均尺寸

50 μm

cleaning

2-13

工作範圍

3-11

分離技術

size exclusion (SEC)

一般說明

HiPrep™ 16/60 Sephacryl® S-300 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 5 mL sample volume. Sephacryl® S-300 is used for separating midsize proteins including antibodies and serum proteins.

特點和優勢

  • Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/ bisacrylamide matrix.
  • Gel filtration columns for low to medium pressure AKTA systems.
  • Well-established in industrial production.
  • The HiPrep™ Sephacryl® series offer reproducible separations over a wide molecular weight range.
  • Convenient, prepacked HiPrep™ Sephacryl® columns speed up protein purification.

適合性

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
Gel filtration columns for low to medium pressure ÄKTA systems.
for preparative separation of biomolecules of up to 5 mL sample volume

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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法律資訊

HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number

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條款

Performing an Isolation of Native Complexes

This page provides information about performing an isolation of natural multi-protein complexes for structural and functional studies using classical biochemical methods and products from Cytiva.

Refolding of proteins using gel filtration

This page shows how to refold proteins using gel filtration and products from Cytiva.

Sephacryl® Separation

Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.

使用Sephacryl®进行分离

本页介绍如何使用Cytiva医疗的sephacryl色谱柱进行分离，该色谱柱是通过将烯丙基葡聚糖与N,N'-亚甲基双丙烯酰胺共价交联而制备的尺寸排阻色谱介质。

相關內容

Performing an Isolation of Native Complexes

This page provides information about performing an isolation of natural multi-protein complexes for structural and functional studies using classical biochemical methods and products from GE Healthcare.

Refolding of proteins using gel filtration

This page shows how to refold proteins using gel filtration and products from GE Healthcare.

Separation Options with Sephacryl®

Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.

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