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GE17-0891-01

Sigma-Aldrich

Percoll 分层液®

Cytiva 17-0891-01, pack of 1 L

同義詞:

Percoll密度梯度介质

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

形狀

colloidal (Colloidal solution of silica coated with polyvinylpyrrolidone (PVP))
liquid (Suspension)
solution

包裝

pack of 1 L

製造商/商標名

Cytiva 17-0891-01

顏色

Colorless to slightly yellow

pH值範圍

8.5-9.5

密度

1.13 g/cm3

相關類別

一般說明

Percoll®是一种良好的参考培养基,用于细胞、病毒和亚细胞颗粒的密度梯度离心。Percoll®是由涂有聚乙烯吡咯烷酮(PVP)的硅胶组成。

Percoll®是一种低黏度密度梯度培养基,用于制备细胞、亚细胞颗粒和较大病毒等。低黏度培养基可通过低离心力(200至1000 x g)在几个分钟内制备预先形成梯度的细胞。该培养基可在易于打开、可重复密封的250 mL和1l瓶中使用。

Percoll®由涂有不可透析聚乙烯吡咯烷酮(PVP)的硅胶颗粒(15至30nm直径)组成。不含PVP的部分仅占1%至2%。Percoll®无毒,几乎呈化学惰性并且不会附着在膜上。Percoll®梯度可在1.0至1.3 g/mL的密度范围内形成,并且在整个过程中等渗透。

Percoll®可在室温下密封保存五年。在-20° C下,仅可保存最多六个月。如果储存于-20°C下,在解冻时会形成梯度,需要在使用前混合瓶子。预先形成的梯度可以储存数周,而不会改变梯度形状,只要保持梯度不冻结。Percoll®可在pH值介于5.5至10.0之间时进行缓冲,而不改变任何特性。如果pH值降至5.5以下,可能会发生凝胶。凝胶也可能由二价阳离子的存在而发生,这种将就会随着温度的升高而加剧。

應用

用于平衡盐溶液、生理盐水或0.25 M蔗糖。

特點和優勢

  • 用于在温和的条件下分离细胞、亚细胞颗粒和更大病毒(下至- 70S),以保持生存能力和形态的完整性。
  • 对细胞无毒。
  • 可调节生理离子强度和pH值。
  • 梯度既可以预先形成,也可以通过在斜角式转头中以中等速度离心自发产生。
  • 梯度在整个过程中等渗透并且其覆盖密度范围高达1.3 g/mL。

儲存和穩定性

储存在4至30 °C ()
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

如需查看本品的分析证书(COA),请访问www.cytiva.com

法律資訊

Percoll is a registered trademark of Cytiva

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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條款

To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.

若要製備 Percoll 梯度,必須使用生理鹽水或細胞培養液調整 Percoll 的滲透性,使 Percoll 與生理鹽溶液呈等張性。

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