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一般說明
GammaBind Plus Sepharose™ is used for lab-scale affinity purification of IgG from a large variety of species and has better binding to rat and mouse IgG subclasses compared to GammaBind Sepharose.
GammaBind Plus Sepharose™ is GammaBind G, Type 3, covalently immobilized to Sepharose™ CL-6B by malimide linkage. GammaBind G, Type 3, a recombinant form of streptococcal protein G, binds to the Fc region of IgG from a variety of mammalian species. GammaBind Plus Sepharose™ may be used to analyze and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium. Since only the Fc region is involved in binding, the Fab region is still available for binding antigen.
GammaBind Plus Sepharose™ is GammaBind G, Type 3, covalently immobilized to Sepharose™ CL-6B by malimide linkage. GammaBind G, Type 3, a recombinant form of streptococcal protein G, binds to the Fc region of IgG from a variety of mammalian species. GammaBind Plus Sepharose™ may be used to analyze and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium. Since only the Fc region is involved in binding, the Fab region is still available for binding antigen.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
特點和優勢
- Recombinant Protein G type 3 coupled to Sepharose™ CL-6B for enhanced binding to rat and mouse IgG subclasses at laboratory scale.
- Binds to all IgG subclasses from human, mouse, and rat; binds total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
- Yields up to 18 mg/mL mouse IgG.
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律資訊
Sepharose is a trademark of Cytiva
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
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