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來源證明/COA

GE17-0033-01

Sephadex^® G-25 Medium

Name: Sephadex<SUP>®</SUP> G-25 Medium
Brand: SIGMA

Cytiva 17-0033-01, pack of 100 g

同義詞:

Gel Filtration Resin

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About This Item

分類程式碼代碼:

41115722

NACRES:

NA.56

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包裝

pack of 100 g

製造商／商標名

Cytiva 17-0033-01

儲存條件

dry media

基質

cross-linked dextran

粒徑

>50 μm (dry)
85-260 μm

cleaning in place

2-13

工作範圍

2-13

適合性

suitable for bioprocess medium

一般說明

Sephadex^® G-25 is well established gel filtration medium for desalting and buffer exchange in industrial applications.

Sephadex^® is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex^® differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex^® G-25 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex^® G-25 is available in 4 different particle sizes (Course, Medium, Fine & Superfine).

Sephadex^® G-25 is available in different bulk pack sizes and convenient pre-packed formats.
As a BioProcess medium, Sephadex^® G-25, meets industrial demands with security of supply and comprehensive technical and regulatory support.

特點和優勢

Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
Excellent recovery and minimum sample dilution
Available in prepacked HiPrep^™ Desalting and HiTrap^® Desalting columns for fast and convenient desalting
BioProcess medium supported for industrial applications and well-established for desalting and buffer exchange in industrial applications

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

HiPrep is a trademark of Cytiva

HiTrap is a registered trademark of Cytiva

Sephadex is a registered trademark of Cytiva

儲存類別代碼

13 - Non Combustible Solids

分析證明 (COA)

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文章

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

抗體親和層析的脫鹽和緩衝液交換

在實驗室規模下進行脫鹽是一種行之有效、簡單且快速的方法，可快速去除低分子量雜質，同時只需一個步驟即可將樣品轉移到所需的緩衝液中。

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Fractional Precipitation Protocol for Affinity Chromatography Samples

This page shows describes fractional precipitation for removing gross impurities from affinity chromatography samples with small volumes.

Performing a Separation with Sephadex^®

This page shows how to perform a separation with a Sephadex column from Cytiva which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.

使用 Sephadex^® 進行分離

本頁說明如何使用 Cytiva 的 Sephadex 色譜柱進行分離，Sephadex 色譜柱是尺寸排阻色譜介質，由表氯醇交聯葡聚糖組成。

親和層析的緩衝液交換和脫鹽

如何使用 Sephadex G-25、HiTrap Desalting columns 或 ÄKTAprime plus 進行緩衝液交換和脫鹽。

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Sephadex^® G-25 Medium