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S5897

Sigma-Aldrich

葡聚糖凝胶 ® G-50

BioReagent, for molecular biology, DNA grade, fine

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
23151817
NACRES:
NA.52

等級

DNA grade
for molecular biology

產品線

BioReagent

形狀

beads

技術

DNA purification: suitable

基質

cross-linked dextran

膨潤

1 g swells to 9-11 mL

珠直徑

20-80 μm

孔徑

exclusion limit (20 bp dsDNA (rA)20)

pH值

2-10

異物活動

DNase, RNase, none detected

儲存溫度

room temp

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

InChI 密鑰

FZWBNHMXJMCXLU-UHFFFAOYSA-N

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一般說明

塞法戴克斯 ® G-50,Fine 是一种常用的凝胶过滤介质,用于 >30,000 MW 生物分子的脱盐和缓冲液交换。塞法戴克斯®通过葡聚糖与环氧氯丙烷交联制备。塞法戴克斯的交联程度不同,因此其溶胀程度和分子分馏范围也不同。“Fine”表示具有极好的效率和较短的扩散距离的小粒径。

S5897-100G 的更新产品编号为 GE17-0573-02

應用

塞法戴克斯 ® G-50 适用于离心柱纯化 DNA(来自污染的小分子),用于杂交。用于 DNA 测序前纯化 PCR 产物,以提高测序质量。

特點和優勢

  • 极佳的回收率
  • 最小样品稀释度
  • 快速且易于使用

法律資訊

Sephadex is a registered trademark of Cytiva

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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存取文件庫

Torben Riehl et al.
PloS one, 7(11), e49354-e49354 (2012-11-13)
The Amundsen Sea, Antarctica, is amongst the most rapidly changing environments of the world. Its benthic inhabitants are barely known and the BIOPEARL 2 project was one of the first to biologically explore this region. Collected during this expedition, Macrostylis
Aron Fazekas et al.
BioTechniques, 48(4), 277-285 (2010-06-24)
Stutter products are a common artifact in the PCR amplification of frequently used genetic markers that contain mononucleotide simple sequence repeats. Despite the importance of accurate determination of nucleotide sequence and allele size, there has been little progress toward decreasing
Mark Del Campo et al.
Journal of molecular biology, 389(4), 674-693 (2009-04-28)
The DEAD-box proteins CYT-19 in Neurospora crassa and Mss116p in Saccharomyces cerevisiae are broadly acting RNA chaperones that function in mitochondria to stimulate group I and group II intron splicing and to activate mRNA translation. Previous studies showed that the
Katharina Besemer et al.
Applied and environmental microbiology, 75(22), 7189-7195 (2009-09-22)
Streams are highly heterogeneous ecosystems, in terms of both geomorphology and hydrodynamics. While flow is recognized to shape the physical architecture of benthic biofilms, we do not yet understand what drives community assembly and biodiversity of benthic biofilms in the
Ryo Takeuchi et al.
Nucleic acids research, 37(3), 877-890 (2008-12-24)
The LAGLIDADG homing endonuclease (LHE) I-AniI has adopted an extremely efficient secondary RNA splicing activity that is beneficial to its host, balanced against inefficient DNA cleavage. A selection experiment identified point mutations in the enzyme that act synergistically to improve

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