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重要文件

G1279

Sigma-Aldrich

G 418 二硫酸盐

powder, suitable for plant cell culture, BioReagent

同義詞:

抗生素G418

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About This Item

經驗公式(希爾表示法):
C20H40N4O10 · 2H2SO4
CAS號碼:
分子量::
692.71
MDL號碼:
分類程式碼代碼:
10171502
PubChem物質ID:
NACRES:
NA.72
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產品名稱

G 418 二硫酸盐, suitable for plant cell culture, BioReagent

產品線

BioReagent

品質等級

形狀

powder

效力

≥720 μg per mg (Dried basis)

技術

cell culture | plant: suitable

顏色

white to off-white

溶解度

100 mg/mL (H2O clear)

抗生素活性譜

Gram-negative bacteria

應用

agriculture

作用方式

protein synthesis | interferes

儲存溫度

2-8°C

SMILES 字串

OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O

InChI

1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1

InChI 密鑰

UHEPSJJJMTWUCP-NKCAIAFTSA-N

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一般說明

Chemical structure: aminoglycoside

應用

G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

注意

The product is stable for three years when stored at 2-8°C.

準備報告

G418 powder is soluble in water at 100 mg/mL.

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

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Frida C Mohlin et al.
Molecular immunology, 65(2), 367-376 (2015-03-04)
Atypical hemolytic uremic syndrome (aHUS) is a disease of complement dysregulation, characterized by hemolytic anemia, thrombocytopenia and acute renal failure. Mutations in complement inhibitors are major risk factors for development of aHUS. The three aHUS patients reported in this study
Yan-Ping Li et al.
STAR protocols, 2(2), 100438-100438 (2021-04-27)
Human-induced pluripotent stem cells (hiPSCs) can be differentiated into well-structured retinal organoids. In this protocol, we successfully established 3D retinae from patient-derived hiPSCs and built the retinitis pigmentosa model in vitro. Moreover, mutation in the retinitis pigmentosa GTPase regulator (RPGR)
Luis M Molinos-Albert et al.
Retrovirology, 11, 44-44 (2014-06-10)
The MPER region of the HIV-1 envelope glycoprotein gp41 is targeted by broadly neutralizing antibodies. However, the localization of this epitope in a hydrophobic environment seems to hamper the elicitation of these antibodies in HIV infected individuals.We have quantified and
Elsa A Marquez et al.
PloS one, 10(7), e0131308-e0131308 (2015-07-07)
Ly49 receptors can be peptide selective in their recognition of MHC-I-peptide complexes, affording them a level of discrimination beyond detecting the presence or absence of specific MHC-I allele products. Despite this ability, little is understood regarding the properties that enable
Glenda Eger et al.
PloS one, 9(9), e109047-e109047 (2014-10-01)
The fibroblast mitogen platelet-derived growth factor -BB (PDGF-BB) induces a transient expression of the orphan nuclear receptor NR4A1 (also named Nur77, TR3 or NGFIB). The aim of the present study was to investigate the pathways through which NR4A1 is induced

文章

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic

抗生素致死曲線是一種劑量反應實驗,哺乳類動物細胞在選擇抗生素時,其劑量會不斷增加。

條款

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

植物組織培養中使用抗生素和抗真菌劑的參考指南和製備指南。

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