E5900

Anti-c-erbB-4 antibody, Mouse monoclonal

clone HER4-36, purified from hybridoma cell culture

• 同義詞: Anti-HER-4
• MDL號碼: MFCD01633326
• 分類程式碼代碼: 51111800
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: HER4-36, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen 180-185 kDa
• 物種活性: human
• 濃度: ~2 mg/mL
• 技術: immunoprecipitation (IP): 4-8 μg/test using RIPA lysate of cultured CB4 cells (CHO cell line transfected with erbB-4); microarray: suitable
• 同型: IgG1
• UniProt登錄號: Q15303
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ERBB4(2066)

描述

一般說明

Monoclonal Anti-c-erbB-4 (erb-b2 receptor tyrosine kinase 4) (HER-4) (mouse IgG1 isotype) is derived from the HER4-36 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a purified extracellular domain of human erbB-4 from transfected cells. The human ErbB-4 oncogene (also known as HER4) is a 180–185 kDa receptor that binds to a family of isoforms, collectively known as neuregulins. This gene belongs to epidermal growth factor receptor family and is expressed mainly in several breast carcinoma cell lines, heart, brain, cerebellum, pituitary and in normal skeletal muscle.

免疫原

extracellular domain of human erbB-4 from transfected cells.

應用

Anti-c-erbB-4 antibody, Mouse monoclonal has been used in immunoprecipitation. It has also been used in studies on receptor turnover, phosphorylation, ligand binding and induction of differentiation of cultured breast cancer cells.

Monoclonal anti-c-erbB-4 antibody can be used in immunoprecipitation (4-8 μg/test) using RIPA lysate of cultured CB4 cells (CHO cell line transfected with erbB-4). It can also be used in microarray.

生化／生理作用

The epidermal growth factor receptor (ErbB/HER) family of transmembrane receptor tyrosine kinases significantly participates in defining cell lineage during mesenchyme-epithelial inductive processes and in the neuro-muscular and glia-Schwann cells interactions. It plays a crucial role as a cell surface receptor for neuregulins. It can also bind with erbB -2 to forms heterodimers and can modulate ligand affinity. The catalytic domain is important for the intrinsic tyrosine kinase activity and shows greater degree of homology. Certain ligands and receptors of the family contribute to a relatively virulent phenotype of some human tumors; most notable are carcinomas of secretory epithelia. ErbB-4 also binds to related group of molecules, termed neuregulin 2 (NRG2)(which also binds to erbB-3) and NRG3 (which binds exclusively to erbB-4). Two other ligands, betacellulin and the heparin-binding EGF-like growth factor, bind to both erbB-1 and erbB-4. ErbB-4 forms heterodimers with erbB-2, resulting in the modulation of its ligand affinity.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable