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生化/生理作用
猪肝酯酶催化酯的对映选择性转化,产生羧酸。分子量为 168kDa。它是一种丝氨酸酶,每个分子上有两个活性位点,它在稀酸或浓盐存在下解离成活性半分子。[6]
酯酶作用于含有短链脂肪酸的水溶性羧酸酯。其功能来自于Ser、His和Asp/Glu的催化三联体。
單位定義
一个酶活性单位是指在pH 8.0、25℃条件下每分钟将1.0 μM丁酸乙酯水解成丁酸和乙醇所需的酶量。
外觀
悬浮于 3.2M(NH4)2SO4 溶液,pH 8
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
從最近期的版本中選擇一個:
分析證明 (COA)
Lot/Batch Number
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文章
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
條款
Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.
在测定酯酶活性时,使用丁酸乙酯通过pH滴定速率法进行测定。酯酶可以将酯水解成酸和醇。
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