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重要文件

D7145

Sigma-Aldrich

2'-脱氧鸟苷 一水合物

99-100%

同義詞:

9-(2-脱氧-β-D-呋喃核糖基)鸟嘌呤, 鸟嘌呤-2′-脱氧核苷

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About This Item

經驗公式(希爾表示法):
C10H13N5O4 · H2O
CAS號碼:
分子量::
285.26
Beilstein:
39814
MDL號碼:
分類程式碼代碼:
41106305
PubChem物質ID:
NACRES:
NA.51

生物源

synthetic (organic)

品質等級

化驗

99-100%

形狀

powder

溶解度

1 M NH4OH: 50 mg/mL, clear, colorless

SMILES 字串

O.NC1=Nc2c(ncn2[C@H]3C[C@H](O)[C@@H](CO)O3)C(=O)N1

InChI

1S/C10H13N5O4.H2O/c11-10-13-8-7(9(18)14-10)12-3-15(8)6-1-4(17)5(2-16)19-6;/h3-6,16-17H,1-2H2,(H3,11,13,14,18);1H2/t4-,5+,6+;/m0./s1

InChI 密鑰

LZSCQUCOIRGCEJ-FPKZOZHISA-N

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應用

2′-脱氧鸟苷一水合物作为核苷补充剂,可用于:
  • 研究其对脱氧尿苷激酶(dguok)突变斑马鱼中线粒体DNA拷贝数的影响
  • 在组织培养基中用于合成脱氧核糖核苷酸(dNTP)
  • 在RPMI(Roswell Park Memorial Institute)-1640培养基中用于去除内源性胸腺细胞

生化/生理作用

脱氧鸟苷(dG)是一种嘌呤核苷,在激酶的连续磷酸化作用下可形成脱氧鸟苷三磷酸(dGTP),dGTP可用于通过DNA聚合酶和逆转录酶合成DNA。在四种经典碱基中,脱氧鸟苷是最富电子的碱基,含有多个易受氧化损伤的亲核位点,因此脱氧鸟苷及其氧化衍生物可用于研究核苷和核苷酸的氧化损伤机制。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

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Zimu Deng et al.
Methods in molecular biology (Clifton, N.J.), 1323, 151-158 (2015-08-22)
Reconstituted thymus organ culture is based on fetal thymus organ culture (FTOC). Purified thymocyte populations, from genetically modified mice or even from other species, are cultured in vitro with thymic lobes depleted of their endogenous thymocytes (by 2'-deoxyguanosine treatment) to
Janna M Mundt et al.
Nucleic acids research, 36(1), 228-236 (2007-11-21)
7,8-Dihydro-8-oxo-2'-deoxyguanosine (8-oxodG) is a well-known marker of oxidative stress. We report a mechanistic analysis of several pathways by which 8-oxodG is converted to nucleotide triphosphates and incorporated into both DNA and RNA. Exposure of MCF-7 cells to [(14)C]8-oxodG combined with
Athanasios Valavanidis et al.
Free radical research, 39(10), 1071-1081 (2005-11-22)
An association between exposure to ambient particulate matter (PM) and increased incidence of mortality and morbidity due to lung cancer and cardiovascular diseases has been demonstrated by recent epidemiological studies. Reactive oxygen species (ROS), especially hydroxyl radicals, generated by PM
Kasper Broedbaek et al.
Free radical biology & medicine, 51(8), 1473-1479 (2011-08-09)
The increasing prevalence of diabetes together with the associated morbidity and mortality calls for additional preventive and therapeutic strategies. New biomarkers that can be used in therapy control and risk stratification as alternatives to current methods are needed and can
Aaron M Fleming et al.
Organic & biomolecular chemistry, 15(39), 8341-8353 (2017-09-25)
In DNA, 2'-deoxyguanosine (dG) is susceptible to oxidative modification by reactive oxygen species (ROS) yielding many products, one of which is 8-oxo-7,8-dihydro-2'-deoxyguanosine (dOG). Interestingly, dOG is stable but much more labile toward oxidation than dG, furnishing 5-guanidinohydantoin-2'-deoxyribose (dGh) that is

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