跳轉至內容
Merck
全部照片(1)

重要文件

D2196

Sigma-Aldrich

DAF-FM solution

5 mM in DMSO, ≥97% (HPLC)

同義詞:

4-Amino-5-methylamino- 2′,7′-difluorofluorescein, Diaminofluorescein-FM

登入查看組織和合約定價


About This Item

經驗公式(希爾表示法):
C21H14F2N2O5
CAS號碼:
分子量::
412.34
MDL號碼:
分類程式碼代碼:
12352200
NACRES:
NA.77
暫時無法取得訂價和供貨情況

品質等級

化驗

≥97% (HPLC)

形狀

DMSO solution

儲存條件

desiccated

顏色

colorless to yellow, including pink

運輸包裝

wet ice

儲存溫度

−20°C

SMILES 字串

Fc1cc2c(cc1O)Oc3c(cc(c(c3)O)F)C42OC(=O)c5c4ccc(c5N)NC

InChI

1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3

InChI 密鑰

DIJCILWNOLHJCG-UHFFFAOYSA-N

應用

DAF-FM solution has been used as a fluorescent probe for detecting nitric oxide (NO) from plant samples.[1]

生化/生理作用

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO).
DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM (Ex: 500 nm; Em: 515 nm; dectection limit: 3 nM) is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, but it does not need to be activated by cytosolic enzymes.

特點和優勢

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


從最近期的版本中選擇一個:

分析證明 (COA)

Lot/Batch Number

未看到正確版本?

如果您需要一個特定的版本,您可以透過批號來尋找特定憑證。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Detection of protein S-nitrosothiols (SNOs) in plant samples on diaminofluorescein (DAF) gels
Rodriguez-Ruiz M, et al.
Bio-protocol null
Marta Rodríguez-Ruiz et al.
Bio-protocol, 7(18), e2559-e2559 (2017-09-20)
In plant cells, the analysis of protein S-nitrosothiols (SNOs) under physiological and adverse stress conditions is essential to understand the mechanisms of Nitric oxide (NO)-based signaling. We adapted a previously reported protocol for detecting protein SNOs in animal systems (

文章

我們提供許多與一氧化氮合酶相關的產品,以滿足您的研究需求。

We offer many products related to nitric oxide synthases for your research needs.

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

一氧化氮 (NO) 是神经元、内皮细胞和免疫系统中的信号转运分子。

Questions

Reviews

No rating value

Active Filters

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務