CMC0023

CONTROLLER SIG10化学感受态细胞

Escherichia coli, rod shaped

• 分類程式碼代碼: 12352200
• NACRES: NA.85

屬性

• product name: CONTROLLER SIG10化学感受态细胞, for controlled protein expression
• 生物源: Escherichia coli
• 等級: for molecular biology
• 增長模式: adherent or suspension
• 形態學: rod shaped
• 技術: microbiological culture: suitable
• 細胞轉化: competent cell type: chemically competent; transformation efficiency: >1 × 10⁹ cfu/μg
• 運輸包裝: dry ice
• 儲存溫度: −70°C

描述

一般說明

The CONTROLLER SIG10 Chemically Competent Cells are an E. coli strain optimized for high efficiency transformation.
They are ideal for cloning and propagation of plasmid clones. They give high yield and high quality plasmid DNA due to the endA1 mutation.

Genotype

mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZΔM15 ΔlacX74 araD139 Δ (ara,leu)7697 galU galK rpsL (StrR) nupG λ- tonA Mini-F lacIq1 (GentR)

特點和優勢

• Reduce leaky expression: The LacI repressor protein binds to the lacO operator to block transcription of downstream genes. In the absence of an induction agent such as lactose sugar or IPTG, target protein expression from the adjacent promoter will be greatly reduced.
• High protein yields: Both 10G and BL21(DE3)-based strains are noted for their ability to express high levels of protein. For expression from a T7 promoter please select the BL21(DE3) strain.

This strain also is deficient in proteases, yielding more stable protein preparations. Most other promoters can be used in either of the CONTROLLER strain.

• Terrific efficiency: The simple, reliable heat-shock procedure for chemical transformation gives excellent results from small amounts of plasmid DNA.

成分

• CONTROLLER SIG10 chemically competent cells
• pUC 19 transformation control DNA
• recovery medium for expression

安全資訊

• 儲存類別代碼: 10 - Combustible liquids