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LB198

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

分類程式碼代碼:
41106514
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生物源

human blood

包裝

tube of 5 μg 98062320-DNA-5UG
pkg of vial of cells 98062320-1VL

增長模式

Suspension

染色體組型

Not specified

形態學

Lymphoblastoid

產品

Not specified

受體

Not specified

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Human lymphocytes, Trichothiodystrophy heterozygote, EBV-transformed

細胞系描述

LB198 has been derived from mononuclear cells from peripheral blood of a 45 year old female. The cells have been transformed with EBV. The patient has been shown to be heterozygote for trichothiodystrophy (TTD) and is the parent of LB199 (Sigma Catalogue number.98062321) TTD is a rare genetic disorder with deficiency in excision repair of UV damage. Fibroblasts from the same individual have been designated TTD HM 1BR.

應用

DNA repair deficiency studies

培養基

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

例行更新培養

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO2, 37°C. Cells form floating aggregates.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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分析證明 (COA)

Lot/Batch Number

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