SVCT

94122105, human breast, Epithelial

• 同義詞: SCTV, SCVT
• 分類程式碼代碼: 41106514

屬性

• 產品名稱: SVCT, 94122105, from human breast
• 生物源: human breast
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice

描述

細胞系來源

Human breast epithelium, SV40 transformed

細胞系描述

SVCT has been established from human breast epithelial cells being transfected with SV40. The cell line shows an abnormal karyotype and was not tumourigenic in athymic male nude mice. SVCT have been found positive for the expression of keratin 7, 8 and 18 but negative for keratin 19. Epithelial cells, dome formation when confluent.

DNA分析

Not specified

培養基

DMEM + 2 mM Glutamine + 5μg/ml Hydrocortisone + 10μg/ml Insulin + 10% Fetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4, i.e., seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

其他說明

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免責聲明

This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.