IPI-2I

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• 分類程式碼代碼: 41106514

屬性

• 生物源: Porcine intestine
• 包裝: tube of 5 μg 93100622-DNA-5UG; pkg of vial of cells 93100622-1VL
• 增長模式: Adherent
• 染色體組型: Heteroploid
• 形態學: Epithelial-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Boar Miniature male ileum, SV40 transformed

細胞系描述

Epitheloid cell line derived from the ileum of an adult histocompatible miniature male boar. Transformed by SV40 plasmid pSV3-neo. Electron microscopy analysis shows evidence of microvilli and desmosomes connecting cells. The cells were found to co-express cytokeratin 18 specific of simple luminal epithelial cells and vimentin associated with a mesenchymal origin. Susceptible to Transmissible Gastro-enteritis (TG) virus.

應用

Studies of epithelial cell differentiation.

培養基

DMEM + 4mM Glutamine + 0.024IU/ml Insulin + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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