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重要文件

293 N3S

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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About This Item

分類程式碼代碼:
41106514
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生物源

human kidney

包裝

tube of 5 μg 92052131-DNA-5UG
pkg of vial of cells 92052131-1VL

增長模式

Suspension

染色體組型

Not specified

形態學

Not specified

產品

Adenovirus 5 early region (E1) antigens

受體

Not specified

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Human embryo kidney

細胞系描述

Adapted for growth in suspension by passaging 293 cells in nude mice. The cells express adenovirus 5 early region 1 (E1) antigens and are permissive for adenovirus 5 host range mutants defective in E1 functions.

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 12,14
D16S539: 9
D5S818: 9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19

培養基

Joklik′s MEM + 2mM Glutamine + 10% Horse Serum.

例行更新培養

Maintain cultures between 5 x 10,000 to 5 x 100,000 cells/ml in a Techne stirrer; 5% CO2; 37°C. Cell doubling time is 30 hours.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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