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BLO-11

90112702, mouse muscle, Fibroblast-like

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About This Item

分類程式碼代碼:
41106514
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產品名稱

BLO-11, 90112702

生物源

mouse muscle

包裝

tube of 5 μg 90112702-DNA-5UG
pkg of vial of cells 90112702-1VL

增長模式

Adherent

染色體組型

2n = 40

形態學

Fibroblast-like

產品

Not specified

受體

Not specified

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Mouse Moblo/y muscle hereditary emphysema

細胞系描述

Established from the abdominal wall muscle of a 30 day old male Blotchy mouse (Mo (blo/y)) with hereditary emphysema. The cells are deficient in lysyl oxidase activity and accumulate high levels of intracellular copper. NOR-10 (Sigma Catalogue number. 90112701) was isolated from the abdominal wall muscle of a normal litter mate of the mouse from which BLO-11 was derived, thus it can serve as a matched control for studies with BLO fibroblasts.

應用

Genetic studies

培養基

DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

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