N1-S1

90011902, rat liver, Epithelial

• 分類程式碼代碼: 41106514

屬性

• product name: N1-S1, 90011902
• 生物源: rat liver
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Rat hepatoma

細胞系描述

Established from the ascitic fluid of a Novikoff hepatoma. The cells can be synchronised for cell cycle studies. N1-S1 has also been used for studies of pyrimidine metabolism, DNA synthesis and methylation and ribosomal RNA maturation.

應用

Cell cycle, metabolic and mutation studies

培養基

Swimm′s S-77 + 2mM Glutamine + 0.1% Pluronic F68 +10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 4x10,000 to 1,000,000 cells/ml; 5% CO₂; 37°C. Subculture every 3-4 days by centrifugation or medium addition.

其他說明

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