Detroit 532

87032602, human skin, Fibroblast

• 分類程式碼代碼: 41106514

屬性

• product name: Detroit 532, 87032602
• 生物源: human skin
• 增長模式: Adherent
• 染色體組型: 2n = 46, A modal chromosome number 47 with trisomy for group 21 in 75% of the cells
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human Caucasian, skin, Down syndrome

細胞系描述

Derived from the foreskin of a 2 month old Caucasian with Down Syndrome.

應用

Virus studies

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan, up to 30 passages expected.

其他說明

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