MM537

12070509, human skin, Epithelial

• 同義詞: MM 537
• 分類程式碼代碼: 41106514

屬性

• 產品名稱: MM537, 12070509, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
• 生物源: human skin
• 包裝: tube of 5 μg 12070509-DNA-5UG; pkg of vial of cells 12070509-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 受體: Not specified
• 相關疾病: metastasis

描述

細胞系來源

Human melanoma; from metastatic site - lymph node

細胞系描述

mM537 is a human melanoma derived from a metastatic site (lymph node). Cell Bank Australia reference number CBA-1356.

DNA分析

Not specified

培養基

RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + 10% FBS.

例行更新培養

Optimal split ratio 1:4 (minimum seeding density 2.7 x 10⁴ cells/cm²). Cells incubated at 37 °C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 1-2 min. MM537 required growth medium to be changed 3 times each week. The cells are sensitive to trypsin so exposure time to trypsin should be kept to a minimum. The cells take 2-3 days to recover from trypsinisation.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.