tube of 5 μg 10092321-DNA-5UG pkg of vial of cells 10092321-1VL
增長模式
Adherent
染色體組型
Not specified
形態學
Epithelial
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
metastasis
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Human melanoma; from metastatic site - lymph node
細胞系描述
Metastatic Melanoma (metastatic site: lymph node). Cell Bank Australia reference number: CBA1345.
培養基
RPMI 1640 (with 2 mM L-Glutamine + 25 mM HEPES) + 10% fetal bovine serum (FBS).
例行更新培養
Optimal split ratio 1:4, MM138 has a relatively slow doubling time of around 60 hours. It does not become confluent and will detach after 6-7 days. If split too hard the doubling time is greatly increased. (Seeding density 0.8 x104 cells/cm2). Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min.
其他說明
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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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分析證明 (COA)
Lot/Batch Number
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