HRA-19a1.1

9071517, human colon, Epithelial

• 同義詞: HRA, HRA19
• 分類程式碼代碼: 41106514

屬性

• product name: HRA-19a1.1, 09071517
• 生物源: human colon
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice

描述

細胞系來源

Human colorectal adenocarcinoma

細胞系描述

The HRA-19 cell line was established from a primary human rectal adenocarcinoma present in a 66-year old male. The adenocarcinoma was well differentiated and determined to be Dukes′ stage B. The HRA-19a1.1 cell line is a clone of HRA-19. HRA-19 has stem cell properties and undergoes a multilineage differentiation in serum free medium.

DNA分析

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 12
D13S317: 12,12
D16S539: 11
D5S818: 11
D7S820: 9,12
THO1: 8
TPOX: 11
vWA: 16

培養基

DMEM - high glucose (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

例行更新培養

Split sub-confluent cultures (70-80%) 1:5 to 1:6 weekly using 0.05% trypsin/EDTA; 7.5% CO₂; 37 °C. Suggested seeding density 2-4x10,000 cells/cm².

其他說明

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免責聲明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.