跳轉至內容
Merck
全部照片(1)

重要文件

C5914

Sigma-Aldrich

非酶细胞裂解液 1x

Prepared in phosphate buffered saline without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture

同義詞:

Dissociation Solution

登入查看組織和合約定價


About This Item

MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.75
暫時無法取得訂價和供貨情況

品質等級

無菌

sterile-filtered

產品線

BioReagent

形狀

liquid

濃度

1 ×

技術

cell culture | mammalian: suitable

pH值

6.9-7.1

運輸包裝

wet ice

儲存溫度

2-8°C

尋找類似的產品? 前往 產品比較指南

一般說明

在人间充质细胞中通过非酶方法进行的细胞分离有助于脱离并保持其功能,特别是细胞表面趋化因子受体的表达。[1]

應用

特殊的非酶配方,用于从塑料或玻璃表面温和地剥离贴壁细胞。
细胞解离液非酶1x已用于视网膜星形胶质细胞培养基、[2] U87胶质母细胞瘤肿瘤细胞[3]和心肌干细胞(CSC)的解离。[4]

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


從最近期的版本中選擇一個:

分析證明 (COA)

Lot/Batch Number

未看到正確版本?

如果您需要一個特定的版本,您可以透過批號來尋找特定憑證。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Ineke D C Jansen et al.
International journal of molecular sciences, 21(17) (2020-08-29)
Lysosome associated membrane proteins (LAMPs) are involved in several processes, among which is fusion of lysosomes with phagosomes. For the formation of multinucleated osteoclasts, the interaction between receptor activator of nuclear kappa β (RANK) and its ligand RANKL is essential.
Non-enzymatic dissociation of human mesenchymal stromal cells improves chemokine-dependent migration and maintains immunosuppressive function
Garg A, et al.
Cytotherapy, 16(4), 545-559 (2014)
Isolation and characterization of murine retinal astrocytes
Scheef E, et al.
Molecular Vision, 11(4), 613-624 (2005)
Human cardiac stem cells with reduced notch signaling show enhanced therapeutic potential in a rat acute infarction model
Matsuda T, et al.
Circulation Journal, 78(1), 222-231 (2014)
Kevin C Osum et al.
Scientific reports, 8(1), 8295-8295 (2018-05-31)
Type 1 diabetes is caused by autoreactive T cell-mediated β cell destruction. Even though co-inhibitory receptor programmed death-1 (PD-1) restrains autoimmunity, the expression and regulation of its cognate ligands on β cell remains unknown. Here, we interrogated β cell-intrinsic programmed

Questions

1–5 of 5 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  2. Can one use Product C5914, Cell Dissociation Solution, with tissue?

    1 answer
    1. We have only used with monolayer cell culture, and therefore we have no information on its usage with tissue.

      Helpful?

  3. Does Product C5914, Cell Dissociation Solution, work with epithelial cells?

    1 answer
    1. It will work with most cell types.

      Helpful?

  4. Does Product C5914, Cell Dissociation Solution, contain EDTA?

    1 answer
    1. Yes, it does contain EDTA; the concentration is proprietary.

      Helpful?

  5. When trypsin is used to detach cells, one usually adds fetal bovine serum (FBS) to the media. Should FBS be used with Product C5914, Cell Dissociation Solution?

    1 answer
    1. No. FBS is used to inactivate trypsin. This product is not enzymatic, so there is no need to use FBS. The cells can be washed with phosphate buffered saline (PBS). When the cells are to be cultured, then one would need to add FBS.

      Helpful?

Reviews

No rating value

Active Filters

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務