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Merck
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文件

C3637

Sigma-Aldrich

Z-L-Lys-ONp hydrochloride

≥98% (HPLC)

同義詞:

N2-[(phenylmethoxy)carbonyl]-L-lysine 4-nitrophenyl ester hydrochloride (1:1) 

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About This Item

經驗公式(希爾表示法):
C20H23N3O6 · HCl
CAS號碼:
分子量::
437.87
EC號碼:
MDL號碼:
分類程式碼代碼:
12352209
eCl@ss:
32160406
PubChem物質ID:
NACRES:
NA.26

product name

Z-L-Lys-ONp hydrochloride,

化驗

≥98% (HPLC)

形狀

powder

顏色

white to off-white

溶解度

methanol: 50 mg/mL, clear, colorless to faintly yellow

儲存溫度

−20°C

SMILES 字串

Cl[H].NCCCC[C@H](NC(=O)OCc1ccccc1)C(=O)Oc2ccc(cc2)[N+]([O-])=O

InChI

1S/C20H23N3O6.ClH/c21-13-5-4-8-18(22-20(25)28-14-15-6-2-1-3-7-15)19(24)29-17-11-9-16(10-12-17)23(26)27;/h1-3,6-7,9-12,18H,4-5,8,13-14,21H2,(H,22,25);1H/t18-;/m0./s1

InChI 密鑰

JFZLPXVXTZKFDV-FERBBOLQSA-N

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應用

Z-L-Lys-ONp hydrochloride has been used as a substrate for trypsin to monitor hydrolysis reaction using mass spectroscopy studies.

生化/生理作用

N-α-carbobenzoxy-L-lysine-p-nitrophenyl ester (Z-L-Lys-ONp) is a para-nitrophenol ester of N-CBZ-L-lysine. Z-L-Lys-ONp and other N-CBZ-amino acid p-nitrophenol esters are chromogenic substrates used for the differentiation and characterization of proteases and endopeptidases. It acts as a substrate for bromelain.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Paul V Attwood et al.
Analytical biochemistry, 334(2), 382-389 (2004-10-21)
Mass spectrometry offers a potential means of measuring virtually all enzyme-catalyzed reactions by simultaneously measuring the concentrations of substrates, products, and intermediates where there are differences in mass between them. To perform these measurements the reaction mixture must be aged
M F Pardo et al.
Biological chemistry, 382(5), 871-874 (2001-08-24)
Unripe fruit extracts of Bromelia balansae Mez (Bromeliaceae), whose principal endopeptidase is balansain I (isolated for anion exchange chromatography: pI = 5.45, molecular weight = 23192), exhibit a pH profile with a maximum activity around pH 9.0 and are inhibited
Susana R Morcelle et al.
Fitoterapia, 75(5), 480-493 (2004-07-21)
As part of a screening of latex endopeptidases from plants growing in Argentina, the presence of proteolytic activity in the latex of Funastrum clausum stems is reported. The proteases present in the crude extract showed the main characteristics of the
P Ascenzi et al.
European journal of biochemistry, 267(4), 1239-1246 (2000-02-15)
Kinetics for the hydrolysis of the chromogenic active-site titrant N alpha-(N,N-dimethylcarbamoyl)-alpha-azaornithine p-nitrophenyl ester (Dmc-azaOrn-ONp) catalysed by bovine beta-trypsin, bovine alpha-thrombin, bovine Factor Xa, human alpha-thrombin, human Factor Xa, human Lys77-plasmin, human urinary kallikrein, Mr 33 000 and Mr 54 000
Constanza Liggieri et al.
Acta biochimica et biophysica Sinica, 41(2), 154-162 (2009-02-11)
Most of the species belonging to Asclepiadaceae family usually secrete an endogenous milk-like fluid in a network of laticifer cells in which sub-cellular organelles intensively synthesize proteins and secondary metabolites. A new papain-like endopeptidase (asclepain c-II) has been isolated and

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