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重要文件

C3260

Sigma-Aldrich

柠檬酸合酶 来源于猪心脏

ammonium sulfate suspension, ≥100 units/mg protein

同義詞:

柠檬酸缩合酶, 柠檬酸草酰乙酸裂解酶

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About This Item

CAS號碼:
酶委員會編號:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54
暫時無法取得訂價和供貨情況

生物源

Porcine heart

形狀

ammonium sulfate suspension

比活性

≥100 units/mg protein

分子量

98 kDa ( 49 kDa monomer)

溶解度

H2O: soluble 1.0 mg/mL, clear

異物活動

isocitrate dehydrogenase and aconitase ≤0.01%
malic dehydrogenase ≤0.1%

儲存溫度

2-8°C

應用

猪心脏来源柠檬酸合酶用于:
  • 注入蝾螈卵子,确定受精时,其在卵子激活中的重要性[1]
  • 制备反应混合物,确定丙酮酸羧化酶活性[2]
  • 检查其是否能够诱导未受精卵中的[Ca22+]增加和卵子激活 [3]

生化/生理作用

柠檬酸合成酶催化柠檬酸在辅酶 A 存在下转化为乙酰辅酶 A,释放 H2O和草酰乙酸。该酶分子量为 85 kDa,pI 为 6.1-6.6。可被氟乙酰辅酶 a、棕榈酰辅酶 a 和柠檬酸辅酶 a 抑制。当其被乙酸酐乙酰化或被碘碘化时也受到抑制。

單位定義

一个装置将形成 1.0μ37°C 下 pH 8.0 时每分钟由草酰乙酸和乙酰 CoA 生成的柠檬酸盐摩尔数。

外觀

用 3.2 M(NH4)2SO4 溶液 (pH 7.0) 混悬。

準備報告

溶于水,形成 1 mg/mL 浓度的澄清溶液。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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文章

Instructions for working with enzymes supplied as ammonium sulfate suspensions

以硫酸铵悬浮液形式提供的酶的使用指南

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