Yes, as mentioned in the product datasheet, the powder form should be stored at –20°C. Stock solutions may be prepared at 100 mg/mL in DMEM/F12 or other culture medium, and stored at –20°C in frozen aliquots. Avoid repeated freeze/thaw cycles. Please see the link below to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/173/127/c2139pis.pdf
C2139
胶原酶 来源于溶组织梭菌
suitable for release of rat epididymal adipocytes and hepatocytes (for methodology see Type II and Type IV), Type VIII, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid
Collagenase from Clostridium histolyticum
同義詞:
梭菌肽酶 A
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關於此項目
CAS 編號:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-582-9
MDL number:
Specific activity:
≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid
Biological source:
Clostridium histolyticum
biological source
Clostridium histolyticum
Quality Segment
type
Type VIII
form
powder
specific activity
≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid
mol wt
68-125 kDa
suitability
suitable for release of rat epididymal adipocytes and hepatocytes (for methodology see Type II and Type IV)
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
Application
Biochem/physiol Actions
每摩尔胶原蛋白酶被4g原子钙活化。 它被乙二醇-双（β-氨基乙基醚）-N，NN′,N′-四乙酸，β-巯基乙醇、谷胱甘肽、巯基乙酸和8-羟基喹啉抑制。
胶原酶由每mol，4g原子钙酶激活。它被乙二醇双(2-氨基乙基醚)四乙酸、β-巯基乙醇、谷胱甘肽、巯基乙酸和8-羟基喹啉所抑制。胶原酶和中性蛋白酶在组织细胞的有效释放中起着重要作用。胶原酶识别序列-R-Pro-8-X-Gly-Pro-R-，其中X表示中性氨基酸。
Analysis Note
也具有梭菌蛋白酶、非特异性中性蛋白酶和胰蛋白酶活性。
Other Notes
一个胶原蛋白消化单元（CDU）从牛跟腱胶原蛋白中释放的肽链量和1.0 μ亮氨酸，5小时，pH7.4,37℃，在钙离子存在下的茚三酮颜色相同。一个 FALGPA水解单元每分钟，25℃，水解1.0 μmole 呋喃基丙烯酰-Leu-Gly-Pro-Ala。一个中性蛋白酶单元水解酪蛋白，产生颜色和1.0 μmole 酪氨酸每5小时，pH7.5,37℃下产生的相同。一个梭菌蛋白酶单元在DTT存在下，pH7.6,25℃，每分钟水解1.0 μmole BAEE。
1 of 1
本產品 | |||
|---|---|---|---|
| description suitable for release of rat epididymal adipocytes and hepatocytes (for methodology see Type II and Type IV), Type VIII, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid | description suitable for release of physiologically active rat epididymal adipocytes, Type II, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid | description 0.2 μm filtered, suitable for release of physiologically active rat hepatocytes, Type IV-S, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid | description Type V, ≥1 FALGPA units/mg solid, >125 CDU/mg solid |
| biological source Clostridium histolyticum | biological source Clostridium histolyticum | biological source - | biological source - |
| specific activity ≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid | specific activity ≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid | specific activity ≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid | specific activity ≥1 FALGPA units/mg solid, >125 CDU/mg solid |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| form powder | form powder | form powder | form powder |
| suitability suitable for release of rat epididymal adipocytes and hepatocytes (for methodology see Type II and Type IV) | suitability - | suitability suitable for release of physiologically active rat hepatocytes | suitability - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
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signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
存儲類別/等級
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Can I use C2139 to make collagenase solution with medium, and then store it at -20?
1 answer-
Helpful?
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is C2139 sterile ?
1 answer-
Product C2139 is not sterile.
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