推薦產品
螢光
λex 546 nm; λem 579 nm
運輸包裝
wet ice
儲存溫度
2-8°C
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一般說明
Baseclick EdU Flow Cytometry Kit为直接检测DNA合成提供了替代BrdU和[3H]thymidine检测的出色方法。EdU（5-乙炔基-2'.-脱氧尿苷）是一种胸苷核苷类似物，在活性DNA合成期间掺入到DNA之中。与BrdU检测法不同，EdU Flow Cytometry Assay不依赖抗体，因而不需进行DNA变性来检测掺入的核苷。恰恰相反，EdU Flow Cytometry Kit利用点击化学法来检测不同种类的染料荧光示值。此外，该试剂盒的实验方案经过简化，减少了总步骤数同时大幅缩减了总耗时。简单的点击化学法检测步骤可在30分钟内完成，在结果的内容和情境丰富程度方面，堪比多重分析方法。
應用
流式细胞术
訊號詞
Danger
危險分類
Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 1B - Repr. 2 - Skin Sens. 1
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Shicheng Su et al.
Cell, 175(2), 442-457 (2018-10-06)
Antibody-dependent cellular cytotoxicity (ADCC) and antibody-dependent cellular phagocytosis (ADCP) critically contribute to the efficacy of anti-tumor therapeutic antibodies. We report here an unexpected finding that macrophages after ADCP inhibit NK cell-mediated ADCC and T cell-mediated cytotoxicity in breast cancers and lymphomas.
Fatemah Chehrehasa et al.
Journal of neuroscience methods, 177(1), 122-130 (2008-11-11)
Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a
Yongmao Yu et al.
Journal of immunological methods, 350(1-2), 29-35 (2009-08-04)
T lymphocyte proliferations can be measured by [(3)H]thymidine incorporation. However, many labs avoid this technique because of the need to use radioactive substrates. In addition, [(3)H]thymidine incorporation method does not permit simultaneous characterization of the proliferating cells. We developed the
相關內容
Experimental studies measuring cell proliferation have had implications in cancer biology, immunology, cell biology, and developmental biology.
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
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