推薦產品
生物源
rabbit
共軛
unconjugated
抗體表格
IgG fraction of antiserum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen ~60 kDa
物種活性
mouse, human
技術
western blot: 1:150-1:300 using whole extracts of mouse 3T3 cells
western blot: 1:250-1:500 using whole extracts of human HeLa cells.
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... KIAA0831(22863)
一般說明
Autophagy-specific subunit of the PtdIns3K complex (Atg14) is encoded by the gene mapped to human chromosome 14q22.3. Atg14, also known as KIAA0831, is an autophagy protein.
特異性
Anti-Atg14 recognizes human and mouse Atg14.
應用
Anti-Atg14 antibody produced in rabbit has been used in western blotting.
生化／生理作用
Autophagy Related 14 (Atg14) is an essential gene for autophagy. Atg14 is present on autophagic isolation membranes and is required for autophagosome formation. Atg14 together with vacuolar protein sorting-associated protein 34 (Vps34) and Atg6/Vps30 forms part of a phosphoinositide 3(PI3)-kinase complex in yeast. Two distinct PI3-kinase complexes, complex I and II, were identified. Complex I is involved in autophagy, whereas complex II is involved in vacuolar protein sorting. In mammals, homologs of Vps34, Vps15, Vps30/Atg6, Atg14 and Vps38 were identified as Vps34, p150, Beclin 1, Atg14 and UV radiation resistance associated gene (UVRAG), respectively. Although both Atg14 and UVRAG interact with Beclin 1, they are not present in the same complex. While Atg14 is present on autophagic isolation membranes, UVRAG mainly localized to Ras-related protein Rab9-positive endocytic compartments.
外觀
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
儲存和穩定性
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Investigation of Androgen Responsive Elements in Some Autophagy Related Genes via In Silico Analysis.
Erzurumlu Y
S Int. Rev. Immunol., 11(1) (2020)
Angiotensin II suppresses autophagy and disrupts ultrastructural morphology and function of mitochondria in mouse skeletal muscle
KAS Silva, et al.
Journal of Applied Physiology, 126(6), 1550-1562 (2019)
Atg14 and UVRAG: mutually exclusive subunits of mammalian Beclin 1-PI3K complexes
Itakura E and Mizushima N
Autophagy, 5(4), 534-536 (2009)
Atg14: a key player in orchestrating autophagy
Obara K and Ohsumi Y
International Journal of Cell Biology, 2011 (2011)
Hong-Min Ni et al.
The American journal of pathology, 183(6), 1815-1825 (2013-10-08)
Autophagy is a lysosomal degradation process that degrades long-lived cellular proteins and damaged organelles as a critical cell survival mechanism in response to stress. We recently reported that acute ethanol induces autophagy, which then reduces ethanol-induced liver injury. However, the
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
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