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Merck
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文件

A4529

Sigma-Aldrich

Aprotinin

3-7 TIU/mg solid, lyophilized powder

同義詞:

BPTI, 抑肽酶, 牛胰胰蛋白酶抑制剂, 胰蛋白酶抑制剂(碱性)

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About This Item

經驗公式(希爾表示法):
C284H432N84O79S7
CAS號碼:
分子量::
6511.44
EC號碼:
MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.54

product name

抑肽酶 来源于牛肺, lyophilized powder, 3-7 TIU/mg solid

生物源

bovine lung

形狀

lyophilized powder

比活性

3-7 TIU/mg solid

分子量

~6,500

溶解度

H2O: >10 mg/mL

UniProt登錄號

儲存溫度

2-8°C

InChI 密鑰

ZPNFWUPYTFPOJU-UHFFFAOYSA-N

基因資訊

cow ... PTI(404172)

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應用

抑肽酶被大量用作胰蛋白酶抑制剂。

生化/生理作用

抑肽酶是一种竞争性丝氨酸蛋白酶抑制剂,可与酶的活性位点组成稳定的复合酶并同时对其进行封闭。 这种结合是可逆的,而且绝大多数抑肽酶-蛋白酶复合物可在极端的pH >10 or < 3水平时发生解离。 从结构上来说,抑肽酶是一种来自牛肺的单体球状蛋白,由58个氨基酸组成并排列在具有三个交联二硫键的单个多肽链中。

單位定義

一个胰蛋白酶抑制剂单位(TIU)可将两个胰蛋白酶单位的活性降低50%,而其中一个胰蛋白酶单位在pH 7.8和25℃下可每分钟水解1.0 μ摩尔的N-α-苯甲酰基-DL-精氨酸对硝基苯胺。另一种常用的单位是KIU,而1 TIU = 1,300 KIU。

準備報告

This product is an affinity purified lyophilized powder purified to remove trace impurities. It has an activity of 3-7 TIU/mg.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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存取文件庫

D Baran et al.
Acta physiologica (Oxford, England), 186(3), 209-221 (2006-02-25)
The renal tubular uptake of 125I-Aprotinin (*Ap) is on average located more superficially than its filtration site, causing transfer of some of *Ap filtered in deep to more superficial cortical zones. 125I-Cystatin C (*Cy) showed less uptake in deep cortical
Hemostatic drugs.
P M Mannucci
The New England journal of medicine, 339(4), 245-253 (1998-07-23)
Birte Treeck et al.
The Journal of physiology, 541(Pt 3), 1049-1057 (2002-06-18)
Different changes in glomerular filtration rates (GFR) in deep and superficial glomeruli have been suggested to influence renal NaCl excretion and concentrating ability. Angiotensin II (AngII) has been implicated in such changes, but the experimental evidence has been conflicting, probably
A M Mahdy et al.
British journal of anaesthesia, 93(6), 842-858 (2004-07-28)
Skilful surgery combined with blood-saving methods and careful management of blood coagulation will all help reduce unnecessary blood loss and transfusion requirements. Excessive surgical bleeding causes hypovolaemia, haemodynamic instability, anaemia and reduced oxygen delivery to tissues, with a subsequent increase
Ilaria Musante et al.
Experimental physiology, 104(6), 866-875 (2019-03-30)
What is the central question of this study? What is the precise subcellular localization of the epithelial sodium channel (ENaC) in human airway epithelium? What is the main finding and its importance? ENaC protein has an unexpected localization in the

文章

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

雖然 aprotinin 與牛胰臟胰蛋白酶抑制劑 (BPTI) 的蛋白質序列相同,但在描述源自牛肺的蛋白質時,通常會使用 aprotinin 一詞。

條款

Objective: To standardize a procedure for the enzymatic assay of Aprotinin.

阿普羅汀是一種牛胰蛋白酶抑制劑。為了測量 aprotinin 的活性,本測試使用 405 nm 的連續分光光度法測定速率。

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