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A1765

Sigma-Aldrich

乙酰辅酶A合成酶 来源于面包酵母(酿酒酵母

lyophilized powder, ≥3 units/mg protein

同義詞:

乙酸硫激酶, 醋酸CoA连接酶(AMP形成)

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.26
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形狀

lyophilized powder

品質等級

比活性

≥3 units/mg protein

成份

Protein, 10-30% biuret

儲存溫度

−20°C

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應用

S-乙酰辅酶 A 合成酶可用于研究各种代谢途径,例如糖酵解、糖异生、丙酮酸代谢和 CO2 固定。它也可以用于基因表达研究。
来自面包酵母的 S-乙酰辅酶 A 合成酶已用于合成 5′-磷酸四磷酸腺苷和 5′-磷酸五磷酸腺苷。[1]

生化/生理作用

乙酰辅酶 A 合成酶催化乙酰辅酶 A 的产生。它参与细胞核中的组蛋白乙酰化。它可能参与不可发酵碳源(如甘油)的生长。乙酰辅酶 A 合成酶是由乙酸盐、乙醛和乙醇诱导的[2]

包裝

包装尺寸基于蛋白质含量。

單位定義

在 37°C,pH 7.5 下,一个单位每分钟将从乙酸盐、ATP 和辅酶 A 生成 1.0 μ 摩尔 S-乙酰辅酶 A。

外觀

冻干粉末,含有稳定剂,如磷酸钾、蔗糖和还原型谷胱甘肽

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Heidi A Crosby et al.
The Journal of biological chemistry, 287(19), 15590-15601 (2012-03-15)
N-lysine acetylation is a posttranslational modification that has been well studied in eukaryotes and is likely widespread in prokaryotes as well. The central metabolic enzyme acetyl-CoA synthetase is regulated in both bacteria and eukaryotes by acetylation of a conserved lysine
Hua Xu et al.
Biochemistry, 50(26), 5883-5892 (2011-06-02)
Recent proteomics studies have revealed that protein acetylation is an abundant and evolutionarily conserved post-translational modification from prokaryotes to eukaryotes. Although an astonishing number of acetylated proteins have been identified in those studies, the acetyltransferases that target these proteins remain
Sandy Thao et al.
mBio, 2(5), doi:10-doi:10 (2011-10-20)
In the bacterium Salmonella enterica, the CobB sirtuin protein deacetylase and the Gcn5-related N(ε)-acetyltransferase (GNAT) Pat control carbon utilization and metabolic flux via N(ε)-lysine acetylation/deacetylation of metabolic enzymes. To date, the S. enterica Pat (SePat) acetyltransferase has not been biochemically
Sara Castaño-Cerezo et al.
Molecular microbiology, 82(5), 1110-1128 (2011-11-09)
Lysine acetylation is a well-established post-translational modification widely conserved and distributed in bacteria. Although multiple regulatory roles have been proved, little is known about its regulation. Here, we present evidence that the transcription of the Gcn5-like acetyltransferase YfiQ of Escherichia
Saurabh Sahar et al.
The Journal of biological chemistry, 289(9), 6091-6097 (2014-01-16)
The circadian clock regulates a wide range of physiological and metabolic processes, and its disruption leads to metabolic disorders such as diabetes and obesity. Accumulating evidence reveals that the circadian clock regulates levels of metabolites that, in turn, may regulate

文章

Enzyme Reagent Coenzyme A (CoA, CoASH or HSCoA) is the key cofactor in first step of the TCA cycle, responsible for transferring the acetyl group from pyruvate oxidation to oxaloacetate yielding citrate. Available through Sigma-Aldrich online.

酵素試劑 輔酵素 A (CoA, CoASH 或 HSCoA) 是 TCA 循環第一步的關鍵輔助因子,負責將丙酮酸氧化產生的乙酰基轉移到草酰乙酸,從而產生檸檬酸。 可透過 Sigma-Aldrich 線上購買。

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