Each kit contains 1.0 mL of DNase I at 1 unit per microliter, totaling about 1000 units per vial. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/708/494/ampd1bul.pdf
Quality Segment
form
liquid
concentration
1 unit/μL
technique(s)
RT-PCR: suitable, whole genome amplification: suitable
color
colorless
shipped in
wet ice
storage temp.
−20°C
General description
当前没有任何 RNA 分离程序可以 100% 去除 DNA。 许多商业DNase I 制剂受到了残留RNase污染， 这种RNase污染会在逆转录以前破坏或分解有价值的RNA样品。实验室比较表明，Sigma′的扩增级 DNase I 的RNase活性低于几家领先的分子生物学产品供应商产品。
脱氧核糖核酸酶I（DNase I）是一种从牛胰腺中分离的内切核酸酶，可将双链和单链DNA消化成寡核苷酸和单核苷酸。 扩增级 DNase I 已纯化去除RNase活性，适于在 RT-PCR（逆转录酶 - 聚合酶链反应）等敏感应用之前去除 RNA 制剂中的DNA。
DNase I可将双链和单链DNA酶解成寡核苷酸和单核苷酸。采用提供的反应缓冲液，在室温下消化 15 分钟，可去除 RNA 制剂中的 DNA。然后随同终止液加热，使DNase I失活。加热还会使RNA中的发夹变性，可直接用于逆转录。
当前没有任何 RNA 分离程序可以 100% 去除 DNA。 许多商业DNase I 制剂受到了残留RNase污染。 这种RNase污染会在逆转录以前破坏或分解有价值的RNA样品。实验室比较表明，Sigma′的扩增级 DNase I 的RNase活性低于几家领先的分子生物学产品供应商产品。
DNase I可将双链和单链DNA酶解成寡核苷酸和单核苷酸。采用提供的反应缓冲液，在室温下消化 15 分钟，可去除 RNA 制剂中的 DNA。然后随同终止液加热，使DNase I失活。加热还会使RNA中的发夹变性，可直接用于逆转录。
当前没有任何 RNA 分离程序可以 100% 去除 DNA。 许多商业DNase I 制剂受到了残留RNase污染。 这种RNase污染会在逆转录以前破坏或分解有价值的RNA样品。实验室比较表明，Sigma′的扩增级 DNase I 的RNase活性低于几家领先的分子生物学产品供应商产品。
Application
适用于去除 RNA 制剂中的 DNA。
Features and Benefits
- 适用于去除 RNA 中的 DNA
- 超低RNase活性
- 经过优化的10×反应缓冲液和终止液，可使DNase I完全失活
Other Notes
在 37 °C 下，一个单位可在 10 min 内将 1μg 质粒DNA 消化为寡核苷酸。
Legal Information
购买本产品附带有限的许可，仅可用于研究用途聚合酶链式反应 (PCR) 过程，且应配合热循环仪使用。该热循环仪在 PCR 过程的自动执行中的使用由预先许可费涵盖，该费用通过向 Applied Biosystems 支付或购买的方式（即授权的热循环仪）支付。
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本產品 | |||
|---|---|---|---|
| description Amplification Grade | description grade II, from bovine pancreas | description from bovine pancreas | description powder, ≥2500 units/g, recombinant, expressed in Aspergillus oryzae |
| technique(s) whole genome amplification: suitable, RT-PCR: suitable | technique(s) DNA extraction: suitable | technique(s) - | technique(s) - |
| concentration 1 unit/μL | concentration - | concentration - | concentration - |
| form liquid | form lyophilized | form dry powder (prepared from crystallized ribonuclease) | form powder |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in - |
| Quality Level 200 | Quality Level - | Quality Level - | Quality Level 200 |
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存儲類別/等級
10 - Combustible liquids
閃點（°C）
Not applicable
閃點 (F)
Not applicable
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Buongiorno vorrei sapere il volume contenuto in una vial di DNAseI cat. AMPD1 1 kit, 1 U/microlitro)
1 answer-
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How can I convert 'units' to mass when adding DNaseI to a buffer solution?
1 answer-
The AMPD1 product, DNase I component D5307, is not tested for protein content, so it is not possible to determine the activity units per mg of protein. This variability in active protein content within a preparation makes it more accurate to sell the product based on its unit activity. If the protocol specifies a certain number of milligrams of DNaseI, it is advisable to determine the specific activity of the enzyme used in the procedure. Alternatively, a titration analysis may be necessary to determine the appropriate amount to use.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What do I do if my DNA is not completely digested after 15 minute digestion at room temperature when using Product AMPD1, DNase I?
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If further digestion is required, incubation can be performed for 30 minutes at 37°C.
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What is the concentration of the DNAse in Product AMPD1, DNase I?
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The DNAse is provided at 1 unit/uL in 1 mL total volume.
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