93862

氯化四唑氮蓝

90.0-110.0% (calc. on dry substance, T), powder

• 同義詞: 氮四唑蓝, 氯化硝基四氮唑蓝, NBT, Nitro BT, 硝基蓝四氮唑, 硝基蓝四氮唑
• 線性公式: C₄₀H₃₀N₁₀O₆ · 2Cl
• CAS號碼: 298-83-9
• 分子量：: 817.64
• Beilstein: 4115923
• MDL號碼: MFCD00012159
• 分類程式碼代碼: 12171500
• PubChem物質ID: 329770281
• NACRES: NA.54

屬性

• 產品名稱: 氯化四唑氮蓝, 90.0-110.0% (calc. on dry substance, T)
• 品質等級: 100
• 化驗: 90.0-110.0% (calc. on dry substance, T)
• 形狀: powder
• 雜質: ≤10% solvent content; ≤10% water
• &lambda ；: in methanol
• 紫外吸收: λ: 252-262 nm A_max
• 儲存溫度: 2-8°C
• SMILES 字串: [Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
• InChI: 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
• InChI 密鑰: FSVCQIDHPKZJSO-UHFFFAOYSA-L

描述

生化／生理作用

Nitrotetrazolium blue chloride (NBT) is used in staining, visualizing and evaluating the damage in brain sections or heart tissues. The principle of the technique is that the redox enzymes like nicotinamide adenine dinucleotide NAD(H) and cofactors present in the cell convert colorless tetrazolium salt to blue formazan. The dead cells remain unstained.[1] It is used in the dual-staining along with methyl green for the hyperspectral microscopic imaging analysis of colon tissues for the diagnosis of colon diseases.[2] NBT is also used for diagnosing microbial infections and phagocytic defects.[3] In conventional chromogenic methods, NBT/ 5-bromo-4-chloro-3-indolyl phosphate (BCIP) is used as a substrate for alkaline phosphatase enzyme reaction, in which NBT is reduced to insoluble purple diformazan.[4]

分析報告

solubility:
250 mg in 5 mL methanol: yellow to very dark yellow and light brown-yellow to very dark brown-yellow

安全資訊

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H302,H315,H319,H335
• 防範說明: P261 - P264 - P270 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• 危險分類: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• 標靶器官: Respiratory system
• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable