88021102

Chang Liver (HeLa derivative) cell line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, human cervix, Epithelial

• 同義詞: Chang Liver cell line (HeLa derivative)
• 分類程式碼代碼: 41106514

屬性

• 生物源: human cervix
• 形狀: liquid
• 包裝: tube of 5 μg 88021102-DNA-5UG; pkg of vial of cells 88021102-1VL
• 增長模式: Adherent
• 染色體組型: Modal no. 70
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer

描述

細胞系來源

Human cervix carcinoma

細胞系描述

The cell line Chang Liver was originally thought to be derived from normal liver. The cell line has since been found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

應用

Chang Liver (HeLa derivative) cell line has been used to study the construction and biological activity of human torque teno virus (TTV) genotype 6.[1]

Virus, biochemical and replication studies

包裝

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12,14
D16S539: 9,10
D5S818: 12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm²using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

免責聲明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable