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86R

Sigma-Aldrich

白细胞碱性磷酸酶试剂盒

based on naphthol AS-BI and fast red violet LB

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About This Item

分類程式碼代碼:
12352106
eCl@ss:
42010105
NACRES:
NA.47
暫時無法取得訂價和供貨情況

品質等級

500

儲存期限

Expiry date on the label.

IVD

for in vitro diagnostic use

dilution

(for histology)

應用

hematology
histology

運輸包裝

wet ice

儲存溫度

2-8°C

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相關類別

應用

将外周血与骨髓样品混合起来制备显微镜玻片。制好的玻片随后在萘酚AS-BI碱性溶液及固红紫色LB的混合液中孵育。所得的不可溶弥散性红色染料沉淀表明了碱性磷酸酶的活性位点。
白细胞碱性磷酸酶 (LAP) 试剂盒用于定性检测白细胞中的碱性磷酸酶活性。

僅套裝組件

產品號碼
描述
  • Citrate Solution (915) 50 mL
  • FRV-Alkaline Solution (862) 10 mL
  • Hematoxylin Solution, Gill No. 3 (kit only) 50 mL
  • Naphthol AS-BI Alkaline Solution (861) 10 mL
  • Sodium Nitrite Solution (914) 10 mL

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2 - STOT RE 2 Oral

標靶器官

Kidney

儲存類別代碼

8A - Combustible corrosive hazardous materials

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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分析證明 (COA)

Lot/Batch Number
0000283230
0000322885
0000251519
SLCM9747
SLCP0230

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Edgardo Rodríguez-Carballo et al.
PloS one, 9(7), e102032-e102032 (2014-07-10)
p38 MAPK activity plays an important role in several steps of the osteoblast lineage progression through activation of osteoblast-specific transcription factors and it is also essential for the acquisition of the osteoblast phenotype in early development. Although reports indicate p38
Alessandro Polini et al.
PloS one, 6(10), e26211-e26211 (2011-10-25)
The development of a new family of implantable bioinspired materials is a focal point of bone tissue engineering. Implant surfaces that better mimic the natural bone extracellular matrix, a naturally nano-composite tissue, can stimulate stem cell differentiation towards osteogenic lineages
Raha Favaedi et al.
The International journal of developmental biology, 60(4-6), 103-110 (2016-07-09)
Histone H3 lysine 9 methylation has been shown to be a critical barrier to efficient cell reprogramming. This discovery allows the assessment of the cell pluripotency state by considering the extent of H3K9 methylation vs. acetylation at the same position.
Terence P Gade et al.
PloS one, 6(7), e22608-e22608 (2011-07-30)
The purpose of this study was to develop a paradigm for quantitative molecular imaging of bone cell activity. We hypothesized the feasibility of non-invasive imaging of the osteoblast enzyme alkaline phosphatase (ALP) using a small imaging molecule in combination with
Clive H Glover et al.
PLoS computational biology, 2(11), e158-e158 (2006-11-24)
Stem cell differentiation involves critical changes in gene expression. Identification of these should provide endpoints useful for optimizing stem cell propagation as well as potential clues about mechanisms governing stem cell maintenance. Here we describe the results of a new
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Questions

1–2 of 2 Questions  

  1. iPS細胞から分化誘導された骨の凍結切片をこのキットで染色すると骨は染まりますか？

    1 answer

    1. The 86C and 86R kits have not been validated for use with frozen sections. The Specimen Collection section of the Instructions For Use advises the kit is intended for "Fresh blood or bone marrow films or samples anticoagulated with heparin may be used. AVOID EDTA." Customers working with cultured cells report the kit does seem to work. This finding has not been confirmed by our company. If the frozen sections have been decalcified, the expectation is that the kit will not work since it is likely the alkaline phosphatase enzyme was destroyed during the decalcification process, There are microtomes that can cut undecalcified bone, although they are not in common use. Really not sure if there are cryostats available that have the ability to cut undecalcified bone. If such a cryostat exists, in theory, the kit might work if the frozen sections are stained immediately after the cutting of the frozen sections. The alkaline phosphatase enzyme is not preserved at -20°C. Were the frozen sections to be stored at -80°C, there is the possibility the alkaline phosphatase enzyme might be preserved. Again there has been no validation for whether the kit can be used to stain frozen sections.

      Helpful?

  2. Can the Alkaline Phosphatase kits in 85 or 85 series kits be used to stain paraffin embedded sections of bone?

    1 answer

    1. The 86R kit, along with its possible replacements (85L1, 85L2, and 86C), is currently not available. These kits, part of the 85 and 86 series, are typically intended for use with peripheral blood and bone marrow. The Instructions for Use advise using fresh blood or bone marrow films anticoagulated with heparin, avoiding EDTA. Blood smears should be stained for enzyme activity within 8 hours after preparation, and films should be dried at least 1 hour before fixation and 3 hours post-fixation before freezing. While these kits are designated for peripheral blood and bone marrow, some users have utilized them for cultured cells and deparaffinized bone. However, the sensitivity of these kits for detecting alkaline phosphatase activity in paraffin sections of bone may be limited.

      Helpful?

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