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Supelco

Atto 565 NHS酯

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

經驗公式(希爾表示法):
C35H34ClN3O11
分子量::
708.11
MDL號碼:
分類程式碼代碼:
12352108
NACRES:
NA.32
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產品線

BioReagent

化驗

≥90% (HPLC)
≥90% (degree of coupling)

形狀

powder

製造商/商標名

ATTO-TEC GmbH

適合性

suitable for fluorescence

檢測方法

fluorometric

儲存溫度

−20°C

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一般說明

Atto 565 NHS 脂是一种常用的胺反应试剂,由 ATTO-TEC 提供。它广泛用于蛋白质标记,且易于与含氨基的化合物(如蛋白质)反应,以在染料之间形成化学稳定的酰胺键,。NHS 脂偶联最佳 pH 值范围为 pH 8.0 - 9.0。在该 pH 值下,蛋白质的氨基基团(比如赖氨酸的 ε-氨基基团)非质子化至足够高以快速耦合。

特點和優勢

  • 强吸收力
  • 高荧光量子产率
  • 高热稳定性和光稳定性

其他說明

生物结合物的荧光 [1] [2];用于荧光共振能量转移,FRET,以及各种实验 [3]

法律資訊

本产品仅供研究使用。如果打算商业化,请联系知识产权持有者(德国ATTO-TEC GmbH公司)申请许可。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Michael Cooper et al.
Journal of fluorescence, 14(2), 145-150 (2004-12-24)
The spectral properties of a rigidified trimethine cyanine dye, Cy3B have been characterised. This probe has excellent fluorescent properties, good water solubility and can be bioconjugated. The emission properties of this fluorophore have also been investigated upon conjugation to an
Elizabeth A Jares-Erijman et al.
Nature biotechnology, 21(11), 1387-1395 (2003-11-05)
Förster (or Fluorescence) Resonance Energy Transfer (FRET) is unique in generating fluorescence signals sensitive to molecular conformation, association, and separation in the 1-10 nm range. We introduce a revised photophysical framework for the phenomenon and provide a systematic catalog of
Benjamin Zoller et al.
Cell, 175(3), 835-847 (2018-10-20)
How transcriptional bursting relates to gene regulation is a central question that has persisted for more than a decade. Here, we measure nascent transcriptional activity in early Drosophila embryos and characterize the variability in absolute activity levels across expression boundaries.
Rachael Bakker et al.
eLife, 9 (2020-06-23)
Morphogen signaling contributes to the patterned spatiotemporal expression of genes during development. One mode of regulation of signaling-responsive genes is at the level of transcription. Single-cell quantitative studies of transcription have revealed that transcription occurs intermittently, in bursts. Although the
Matthew G Niepielko et al.
Current biology : CB, 28(12), 1872-1881 (2018-06-05)
The formation of ribonucleoprotein assemblies called germ granules is a conserved feature of germline development. In Drosophila, germ granules form at the posterior of the oocyte in a specialized cytoplasm called the germ plasm, which specifies germline fate during embryogenesis. mRNAs

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致色劑和致螢光劑衍生物是生物化學的寶貴工具,在酵素學、蛋白質化學、免疫學和組織化學方面有許多應用。

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