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Merck
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69898

Sigma-Aldrich

溴乙烷

suitable for fluorescence, BioReagent, ≥95% (HPCE)

同義詞:

单溴代二苯甲烷

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About This Item

經驗公式（希爾表示法）:
C10H11BrN2O2
CAS號碼:
71418-44-5
分子量：:
271.11
Beilstein:
4430959
MDL號碼:
MFCD00036951
分類程式碼代碼:
12171500
PubChem物質ID:
57652223
NACRES:
NA.32

產品線

BioReagent

化驗

≥95% (HPCE)

形狀

solid

mp

152-154 °C (lit.)

溶解度

DMF: soluble
DMSO: soluble
acetonitrile: soluble
methanol: soluble

螢光

λex 390 nm; λem 478 nm in 0.1 M phosphate pH 7.5 (after derivatization with glutathione)
λex 398 nm

適合性

suitable for fluorescence

儲存溫度

2-8°C

SMILES 字串

CC1=C(C)C(=O)N2N1C(CBr)=C(C)C2=O

InChI

1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3

InChI 密鑰

AHEWZZJEDQVLOP-UHFFFAOYSA-N

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相關類別

一般說明

溴乙烷也被称为硫醇探针，是一种通过光解反应激活的荧光试剂。溴乙烷在溶液中与小硫醇基团（比如 GSH）和有反应活性的蛋白硫醇基团（如 血红蛋白）反应。 溴乙烷与硫醇的反应是二级反应，和pH相关。并且，通过与硫醇反应，激活了用于检测的水溶性荧光物质。

應用

溴乙烷被用于通过HPLC测定硫醇。 它适用于作为荧光测定2,3-二巯基丙烷-1-磺酸和其他二硫醇的柱前衍生试剂。 溴乙烷被用做研究牛心脏线粒体中对寡霉素敏感的ATP酶的荧光标记。

特點和優勢

用溴乙烷分析低分子量硫醇的好处在于： 高选择性。
  • 反应快速。
  • 易于通过反相HPLC分离衍生物。
  • 能穿透细胞。

包裝

无底玻璃瓶。内含物在插入的融合锥体内。

其他說明

荧光硫醇特异性试剂

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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R M Maiorino et al.
Journal of chromatography, 374(2), 297-310 (1986-01-24)
The increasing therapeutic use of dithiol metal binding agents, such as 2,3-dimercaptopropane-1-sulfonic acid (DMPS), has stimulated the need for a sensitive and selective method for their determination in biological fluids. A method has now been developed in which DMPS was
Determination of low-molecular-weight thiols using monobromobimane fluorescent labeling and high-performance liquid chromatography.
R C Fahey et al.
Methods in enzymology, 143, 85-96 (1987-01-01)
P C Chinn et al.
Analytical biochemistry, 159(1), 143-149 (1986-11-15)
A highly sensitive and specific assay for Escherichia coli thioredoxin was developed using the thiol-specific reagent monobromobimane. Treatment of dithiothreitol-reduced thioredoxin with an excess of monobromobimane in Tris buffer (pH 8.0, 23 degrees C) for 30 min resulted in the
Edward A Wintner et al.
British journal of pharmacology, 160(4), 941-957 (2010-07-02)
Hydrogen sulphide (H(2)S) is a labile, endogenous metabolite of cysteine, with multiple biological roles. The development of sulphide-based therapies for human diseases will benefit from a reliable method of quantifying H(2)S in blood and tissues. Concentrations of reactive sulphide in
Michael Hall et al.
Proteomics, 10(5), 987-1001 (2010-01-06)
The light-dependent regulation of stromal enzymes by thioredoxin (Trx)-catalysed disulphide/dithiol exchange is known as a classical mechanism for control of chloroplast metabolism. Recent proteome studies show that Trx targets are present not only in the stroma but in all chloroplast
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