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重要文件

64958

Sigma-Aldrich

2-甲氧基-2,4-二苯基-3(2H)-呋喃酮

suitable for fluorescence, ≥98.0% (HPLC)

同義詞:

2,4-Diphenyl-2-methoxy-3(2H)-furanone, MDPF

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About This Item

經驗公式(希爾表示法):
C17H14O3
CAS號碼:
分子量::
266.29
Beilstein:
1288529
MDL號碼:
分類程式碼代碼:
12352108
PubChem物質ID:
NACRES:
NA.32
暫時無法取得訂價和供貨情況

化驗

≥98.0% (HPLC)

形狀

solid

螢光

λex 384 nm; λem 472 nm in acetonitrile (after derivatization with hexylamine)

適合性

suitable for fluorescence

儲存溫度

2-8°C

SMILES 字串

COC1(OC=C(C1=O)c2ccccc2)c3ccccc3

InChI

1S/C17H14O3/c1-19-17(14-10-6-3-7-11-14)16(18)15(12-20-17)13-8-4-2-5-9-13/h2-12H,1H3

InChI 密鑰

BLWINLJDTOJSRU-UHFFFAOYSA-N

應用

2-Methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) is used as a fluorescence reagent to derivatize primary and secondary amines on molecules such as proteins. MDPF derivatized molecules can be detected during analytical and separation procedures including chromatography, electrophoresis and zymography.

其他說明

Reagent for the derivatization of primary and secondary amines for HPLC; highly fluorescent derivatives are formed[1][2]; Pre-column derivatization of amines[3]; Fluorescent labeling of proteins before SDS-PAGE[4]

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


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分析證明 (COA)

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R C Schaeffer et al.
International journal of microcirculation, clinical and experimental, 11(2), 181-201 (1992-05-11)
We studied the macromolecular size-selective transport characteristics of polycarbonate (PC) filters with defined pore radius (rp; 15,000 to 400 A) as well as 2,000 A rp PC filter-bovine pulmonary artery endothelial cell (EC) monolayer sandwich under zero hydrostatic pressure conditions
Danqing Min et al.
Electrophoresis, 27(2), 357-364 (2005-12-07)
Measurement of matrix metalloproteinases (MMPs) and their specific tissue inhibitors of metalloproteinases (TIMPs) by the techniques of zymography and reverse zymography provide useful information regarding the status of matrix accumulation or breakdown. This report describes the use of 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF)
P Jackson et al.
Electrophoresis, 9(7), 330-339 (1988-07-01)
A new method for visualising proteins in two-dimensional polyacrylamide gels was developed. Proteins were labelled with the fluorophore 2-methoxy-2,4-diphenyl-3(2H)furanone (MDPF) while present in the first-dimensional gel after isoelectric focusing and subsequently electrophoresed into the second-dimensional gel. High resolution spot patterns
B W Falk et al.
Analytical biochemistry, 144(2), 537-541 (1985-02-01)
Fluorescent labeling of proteins was found to be a very sensitive and reliable alternative to conventional methods for monitoring proteins on Western blots. Proteins were labeled with 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) before SDS-PAGE. After electrophoresis and subsequent electro-blotting the fluorescent-labeled proteins were
S K Williams et al.
Journal of cellular physiology, 120(2), 157-162 (1984-08-01)
The transport of proteins across continuous capillary endothelium is believed to be mediated by micropinocytic vesicles which shuttle molecules between the lumenal and abluminal plasma membrane. We have studied the ability of capillary endothelial cells isolated from rat epididymal fat

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