388M-1

Nestin (10C2) Mouse Monoclonal Antibody

• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100; 500
• 共軛: unconjugated
• 抗體表格: culture supernatant
• 抗體產品種類: primary antibodies
• 無性繁殖: 10C2, monoclonal
• 描述: For In Vitro Diagnostic Use in Select Regions (See Chart)
• 形狀: buffered aqueous solution
• 物種活性: human
• 包裝: vial of 0.1 mL concentrate (388M-14); vial of 0.5 mL concentrate (388M-15); bottle of 1.0 mL predilute (388M-17); vial of 1.0 mL concentrate (388M-16); bottle of 7.0 mL predilute (388M-18)
• 製造商／商標名: Cell Marque^®
• 技術: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100
• 同型: IgG1
• 控制: tonsil
• 運輸包裝: wet ice
• 儲存溫度: 2-8°C
• 視覺化: cytoplasmic
• 基因資訊: human ... NES(10763)

描述

一般說明

Nestin is a class VI intermediate filament (IF) protein. It has been reported that nestin expression is significantly increased in melanoma and correlated with more advanced stages of the disease.[1] An immunohistochemical analysis identified nestin-positive cells in 84% (35/42) of primary melanoma and 83% (10/12) of metastatic melanoma.[1] Nestin immunoreactivity was also observed in melanoma cells in all (10/10) cases of HMB-45-negative amelanotic and melanotic, non-desmoplastic melanoma.[2] Nestin expression has been reported in tumors of the CNS, including astrocytoma, ependymoma, oligodendroglioma, glioblastoma, and primitive neuroectodermal tumors.[3] Nestin has been detected in human gliomas, including low and high grade, but its expression is observed more frequently in high grade than in low grade gliomas.[3] Nestin overexpression is seen in carcinomas, including prostatic adenocarcinoma, pancreatic ductal carcinoma, thyroid carcinoma, and in mesenchymal tumors such as GIST and DFSP.[3] Among the breast carcinoma subtypes, nestin is highly expressed in basal breast cancer subtype (ERα-/PR-/Her2-) but not in the Her2 subtype (ERα-/PR-/Her2+) or luminal epithelial phenotype (ERα+/PR+).[4] Only cytoplasmic staining is considered positive, whereas any nuclear staining is considered as background artifact. In normal skin, nestin is observed in endothelial cells and the bulge area of hair follicles.

品質

IVD

IVD

IVD

RUO

聯結

Nestin Positive Control Slides, Product No. 388S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

外觀

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

準備報告

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

其他說明

For Technical Service please contact: 800-665-7284 or email: [email protected]

法律資訊

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany