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Merck
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14365C

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EX-CELL® CD CHO Fusion

Animal-component free, without L-glutamine, without hypoxanthine, without thymidine, liquid, sterile-filtered, suitable for cell culture

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同義詞:

EX-CELL® CD CHO Fusion

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.75

品質等級

500

無菌

sterile-filtered

產品線

EX-CELL®

形狀

liquid

技術

cell culture | mammalian: suitable (suspension)

成分

L-glutamine: no

運輸包裝

ambient

儲存溫度

2-8°C

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相關類別

一般說明

EX-CELL® CD CHO FUSION 是一种化学成分确定、不含动物组分的培养基， 是为了中国仓鼠卵巢（CHO）细胞的长期生长而开发。 该 不含任何大分子，所以可用于分离和纯化 细胞 分泌的蛋白质。该培养基不含 L-谷氨酰胺， 这有助于培养基的稳定，避免了L-谷氨酰胺降解所导致的 氨积累， 并为以谷氨酰胺合成酶选择性进行的CHO 细胞培养 （如：MilliporeSigma的CHOZN GS 敲除细胞系）提供合适的培养基。该培养基不含次黄嘌呤或 胸苷 ，因此可以用于二氢叶酸还原酶（DHFR）基因扩增 系统（如：MilliporeSigma的CHOZN DHFR敲除细胞系）

數量

按照 每升培养基20.9克粉末配制。

法律資訊

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

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Noriko Yamano-Adachi et al.
Scientific reports, 10(1), 17612-17612 (2020-10-21)
Chinese hamster (Cricetulus griseus) ovary-derived Chinese hamster ovary (CHO) cells are the most commonly used mammalian hosts for the industrial production of recombinant therapeutics because of their ability to fold, assemble, and perform post-translational modifications, such as glycosylation, on proteins.
Qiong Wang et al.
Biotechnology and bioengineering, 116(9), 2303-2315 (2019-05-08)
With the increasing demand to provide more detailed quality attributes, more sophisticated glycan analysis tools are highly desirable for biopharmaceutical manufacturing. Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the
Weiyi Liu et al.
Biotechnology progress, 37(1), e3061-e3061 (2020-08-05)
Antibody-dependent cellular cytotoxicity (ADCC) is the primary mechanism of actions for several marketed therapeutic antibodies (mAbs) and for many more in clinical trials. The ADCC efficacy is highly dependent on the ability of therapeutic mAbs to recruit effector cells such

文章

Operational and Financial Advantages of Cellicon® Cell Retention Solution in an N-1 Perfusion Process

This page describes the financial and operational advantages offered by the Cellicon® Cell Retention Device in an N-1 perfusion process at the 2,000 L scale in comparison to a cell retention device from another supplier.

通过灌流式种子培养优化CHO细胞制备，以提高生产力

本应用说明描述了Cellvento® 4CHO-X细胞扩增培养基的优势和性能，这款培养基专门开发用于从细胞复苏到N-1阶段的多个细胞扩增步骤，以生成更多生物质接种补料分批生产生物反应器。

Cell Retention Technology and Specialized Cell Culture Media for Intensified Upstream Processing

How the Cellicon® filter and controller enables seamless adoption of a perfused N-1 seed train step into the cell expansion process.

Optimizing CHO Cells for Higher Productivity

Cellvento 4CHO-X Expansion Medium enhances cell biomass for bioreactor inoculation in cell expansion stages.

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