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重要文件

03978

Sigma-Aldrich

亚甲蓝 溶液

for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

同義詞:

亚甲蓝染剂, 吕弗勒美蓝, 欧利希试剂 III

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About This Item

經驗公式(希爾表示法):
C16H18ClN3S
CAS號碼:
分子量::
319.85
Beilstein:
3641570
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47
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等級

for microscopy

形狀

liquid

品質

concentrated, aqueous solution

儲存期限

~3 yr

濃度

1.5% (1.5 g/ 100 mL)

密度

1.0 g/mL at 20 °C

εmax

9.4 at 656-666 nm in water

適合性

suitable for microscopy

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

room temp

SMILES 字串

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI 密鑰

CXKWCBBOMKCUKX-UHFFFAOYSA-M

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一般說明

亚甲蓝也称为氯化四甲基硫堇,是一种光敏感型酚噻嗪染料。[1]它是一种小分子量染料,[2]可有效抵抗革兰氏阳性和革兰氏阴性菌侵袭。[3]
亚甲蓝是用来染色动物细胞,使其细胞核更容易观察。是瑞氏染色和詹纳染色等染色介质的成分。

應用

亚甲蓝溶液已用于对人羊水干细胞进行染色以测定细胞活性。[4]它还用于切片染色以确定分枝杆菌的存在。[5][6]
亚甲蓝用于染色细胞学中使用的血膜/涂片,以及用于染色在显微镜下或杂交膜上观察的 RNA 或 DNA。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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分析證明 (COA)

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Questions

  1. Concerning the stated concentration of the product 03978 (Methylene blue solution, 1.5%), what is the uncertainty and lot-to-lot variability?

    1 answer
    1. Product 03978 is not a GMP quality product. No testing is performed during Quality Control testing that measures lot to lot variability. The testing includes tests for Appearance, Thin Layer Chromatography, Density, Wavelength 1 at 656-666nm, Extinction Coefficient for Wavelength 1 and the solvent used to measure the wavelength and extinction coefficient. The information about a 1.5% concentration of dye is from the production documents used to manufacture the dye solution. The actual dye concentration is not measured.

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