PPB013

1.0% Agarose in Tris-Borate EDTA Buffer

pHast Pack^™, powder

• 同義詞: 1% Agarose gel, TAE Agarose blend, TBE Agarose blend, TBE buffer garose blend
• 分類程式碼代碼: 41105317
• NACRES: NA.25

屬性

• 產品線: pHast Pack^™
• 品質等級: 100
• 形狀: powder
• pH值: 8.1-8.5(range for the Tris Borate ETDA buffer prior to blending with agarose)
• 正離子痕跡: Pb: ≤5 ppm
• 異物活動: DNase, none detected; Endonuclease ; Exonuclease, none detected; NICKase, none detected; RNase, none detected

描述

一般說明

1% Agarose in Tris Borate EDTA (TBE buffer, pH 8.3) is a powder blend that dissolves easily in boiling water to quickly make an agarose gel for electrophoresis. Typically, 1% Agarose gel can separate DNA or RNA fragments between a range of 300 – 7,000 bp

應用

1% Agarose gel in 1X TBE buffer may be used for:

• DNA electrophoresis
• Native RNA Electrophoresis

特點和優勢

• Ready-to-pour 1% agarose gel eliminates buffer prep
• Accelerates workflow and minimize cost and labor
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm Lead ≤5 ppm
• Compatible with TBE pHast pack^™ buffer

包裝

Foil pouches

準備報告

Each packet prepares 250mL of molten agarose that is typically used to cast horizontal gels. A small electrophoresis apparatus may use 30 - 50mL, medium 100mL, and large rigs 250mL. Add nucleic acid stain before pouring the gel. For optimal results pair with pHast pack running buffer 1X TBE, pH 7.3.

重構

Contents of one pouch, when dissolved in 250 mL of ultrapure water, will yield a 1× solution containing 89 mM Tris borate, 2 mM EDTA, and 1.0% (w/v) agarose. Certified to be DNAse, RNAse, and Nickase free. Prepare in a 1L flask. Combine pHast pack contents with 250 mL ultrapure water and mix well. Microwave on high for ~1min to boil and dissolve the agarose. Use a heat resistant glove to remove the flask every 10 - 15s to mix gently by swirling the liquid in a circle at the bottom of the flask. Repeat until the agarose is fully dissolved. Cool the flask slightly with cold water while swirling every 10 – 15s, add the stain and pour the gel. Allow agarose gel to fully cool and solidify before removing combs.

儲存和穩定性

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

其他說明

For additional information on our range of Biochemicals, please complete this form.

法律資訊

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

安全資訊

• 象形圖: GHS08
• 訊號詞: Danger
• 危險聲明: H360FD
• 防範說明: P201 - P202 - P280 - P308 + P313 - P405 - P501
• 危險分類: Repr. 1B
• 儲存類別代碼: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• 水污染物質分類（WGK）: WGK 1