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重要文件

C2734

Sigma-Aldrich

5-羧基四甲基罗丹明

≥95% purity (HPLC), solid

同義詞:

5-TAMRA

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About This Item

經驗公式(希爾表示法):
C25H22N2O5
CAS號碼:
分子量::
430.45
MDL號碼:
分類程式碼代碼:
12171500
NACRES:
NA.47
暫時無法取得訂價和供貨情況

產品名稱

5-羧基四甲基罗丹明,

化驗

≥95% (HPLC)

品質等級

形狀

solid

溶解度

water: slightly soluble

&epsilon ;(消光係數)

≥85000-97000 at 539-545 nm in methanol

螢光

λex ~543 nm; λem ~570 nm in methanol

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

room temp

SMILES 字串

N(C)(C)c1cc2[o+]c3c(c(c2cc1)c4c(cc(cc4)C(=O)[O-])C(=O)O)ccc(c3)N(C)C

InChI

1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)

InChI 密鑰

YMZMTOFQCVHHFB-UHFFFAOYSA-N

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一般說明

在高纯度中制备的重要荧光基团,通过约 600 个分子的毛细管电泳检测限为 TMR 标记氨基酸。容易被荧光显微镜中使用的汞弧灯的 546 nm 光谱线激发,本质上比荧光素更具光稳定性。

應用

5-羧基四甲基罗丹明已用于荧光团表征。[1]

生化/生理作用

5-羧基四甲基罗丹明可以共价连接到 DNA 上,从而用于涉及分支和双链 DNA/RNA 结构的研究。[2]主要用于单分子研究。[3]5-羧基四甲基罗丹明也用于标记寡糖,用于激光诱导荧光检测。[4]

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Limary M Cancel et al.
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Dustin J Paul et al.
Nucleic acids research, 37(17), 5894-5907 (2009-09-04)
Herein, we report the identification of RNA hairpin loops that bind derivatives of kanamycin A, tobramycin, neamine, and neomycin B via two-dimensional combinatorial screening, a method that screens chemical and RNA spaces simultaneously. An arrayed aminoglycoside library was probed for
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Pimenta S, et al.
Journal of Biomedical Optics, 23(10), 105004-105004 (2018)

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