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Merck
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重要文件

A7409

Sigma-Aldrich

白蛋白 溶液 来源于牛血清

35% in 0.85% sodium chloride, aseptically filled

同義詞:

BSA溶液

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About This Item

CAS號碼:
9048-46-8
MDL號碼:
MFCD00130384
分類程式碼代碼:
12352202
NACRES:
NA.27
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生物源

bovine

品質等級

300

描述

contains no preservative

無菌

aseptically filled

形狀

solution

純化經由

heat shock fractionation

來源

USA origin

濃度

33.0-37.4% protein (biuret)
35% in 0.85% sodium chloride

技術

microbiological culture: suitable

UniProt登錄號

P02769

儲存溫度

2-8°C

基因資訊

bovine ... ALB(280717)

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相關類別

一般說明

Albumin belongs to the four-member albumin superfamily.[1] It is a major protein in the circulating blood.[2] Albumin has a helical structure with three domains that form a heart-like shape.[1]

應用

Bovine Serum Albumin solution has been used:
  • to block protein-coated well plates in enzyme-linked immunosorbent assay (ELISA) for immunoreactivity and cross-reactivity studies in the production of monoclonal antibodies (mAbs)[3]
  • to prepare primary antibody solution for peroxidases staining[4]
  • as a supplement for in vitro cultivation of Borrelia strains[4]

生化／生理作用

Bovine serum albumin (BSA) serves as a protein supplement for cell culture media.[5] BSA acts as a transport carrier for drugs, and it is commonly used to study the protein-drug interactions. Furthermore, it is known for its ability to interact with several aromatic and hydrophobic molecules of the bloodstream.[6]

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves

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分析證明 (COA)

Lot/Batch Number
0000425199
0000425199
0000374288
0000386367
0000384626

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存取文件庫

Fatai S Oladunni et al.
Journal of virological methods, 290, 114084-114084 (2021-01-30)
The use of monoclonal neutralizing antibodies (mNAbs) is being actively pursued as a viable intervention for the treatment of Severe Acute Respiratory Syndrome CoV-2 (SARS-CoV-2) infection and associated coronavirus disease 2019 (COVID-19). While highly potent mNAbs have great therapeutic potential
Bovine serum albumin
Meyler's Side Effects of Drugs: The International Encyclopedia of Adverse Drug Reactions and Interactions, 1045 null
Ines Martin-Martin et al.
Cold Spring Harbor protocols, 2022(10), Pdb-Pdb (2022-08-13)
Immunohistochemistry is a valuable technique that provides information on protein localization and interactions in tissues. Mosquito salivary gland immunohistochemistry requires the meticulous dissection of a delicate tissue. The integrity of the salivary glands must be closely monitored throughout the entire
Sabrina Hepner et al.
Ticks and tick-borne diseases, 10(5), 1157-1161 (2019-06-27)
Borrelia turcica is a reptile-associated Borrelia species that is vectored by the hard tick Hyalomma aegyptium. Tortoises of the genus Testudo represent the principal host of adult H. aegyptium, while immature stages are less host-specific and can be found on
Paul A Wilson et al.
PloS one, 16(10), e0258316-e0258316 (2021-10-22)
RORγt is an isoform of RORC, preferentially expressed in Th17 cells, that functions as a critical regulator of type 3 immunity. As murine Th17-driven inflammatory disease models were greatly diminished in RORC knock-out mice, this receptor was prioritised as an
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Questions

  1. Is the BSA cat. A7409 suitable for MACS buffer used for T cell separation? Separated cells would be cultured for around 2 weeks

    1 answer

    1. This product has not been tested for suitability in this application. While it is aseptically prepared, this product is not tested for sterility.

      See product A7979 for the sterile, cell culture tested BSA solution that may be more suitable: https://www.sigmaaldrich.com/product/sigma/a7979

      In addition, see product A9418 to review another cell culture tested product that is cited for use in MACS buffer: https://www.sigmaaldrich.com/product/sigma/a9418

      A link to this publication is below:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4059065/

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