It is possible that either too much glacial acetic acid was added, or the acetic acid was added too quickly. When using the 0.5% Eosin Y solution sold as catalog number HT110216-500 ml, the instructions recommend adding 0.5 glacial acetic acid slowly to 100 ml of stain solution just prior to use. It's not uncommon to observe this in the lab. In most cases, the precipitate can be resolved by swirling the solution by hand or mixing it on a stir plate.
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About This Item
經驗公式（希爾表示法）:
C20H6Br4Na2O5
CAS號碼:
分子量：:
691.85
Beilstein:
1443944
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47
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推薦產品
形狀
liquid
品質等級
濃度
5 wt. % in H2O
顏色
red to very dark red
密度
1.015 g/mL at 25 °C
λmax
518 nm
應用
diagnostic assay manufacturing
hematology
histology
儲存溫度
room temp
SMILES 字串
[Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24
InChI
1S/C20H8Br4O5.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
InChI 密鑰
SEACYXSIPDVVMV-UHFFFAOYSA-L
尋找類似的產品？ 前往 產品比較指南
一般說明
曙红Y也称曙红微黄，是一种广泛使用的氧杂蒽染料。它是一种复染剂，用于区分各种结缔组织纤维&基质和不同类型细胞的细胞质。曙红与苏木精同用可显示组织的一般组织学结构。
應用
曙红 Y 适用于苏木精和曙红 (H&E) 染色流程，可用于各种组织样品，如肺结节和肾切片。
訊號詞
Warning
危險聲明
危險分類
Skin Sens. 1
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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a 1% Eosin Y solution in water using the product under the catalog number 318906 (Eosin Y solution 5wt% in water) was created and then diluted to 1% in water, the pH was lowered with acetic acid, but it immediately changed to a bright red color and produced a precipitate?
1 answer-
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Product 318906 suitable for use in Hematoxylin and Eosin (H&E) staining procedures?
1 answer-
Product 318906 was not intended for use with H&E staining techniques. The dye content for the Eosin Y is 5% in water. For H&E staining, the concentration is typically 0.5 to 1.0 %. At 5 % dye content, the dye is much too concentrated for use with H&E staining. If used at such a high concentration, the cytoplasmic staining is likely to obscure the nuclear staining from the hematoxylin. A well stained H&E slide should show blue nuclear staining and typically at least 3 shades of Eosin staining. When used at 5% dye content, the cytoplasmic staining is likely to be a homogenous or monotone.The 5% Eosin solution has only one common application - determining sperm viability. In a typical semen analysis, 5% Eosin Y is used in conjunction with 10% Nigrosin. This combination of dyes is used to judge the viability of the sperm. A standard H&E procedure is then used to judge the morphology of the sperm. Sperm counts are typically performed without the use of a dye solution.It is possible to consider a 5% Eosin solution as a 10X staining solution. The product, however, is not suitability tested for use in either a sperm viability procedure or a H&E staining procedure.The following products have been suitability tested for use in H&E staining procedures and are considered suitable for use in In Vitro Diagnostic procedures. They are the preferred products for H&E staining. Most are formulated with 0.5% Eosin Y. The exception is Eosin Y with Phloxine B. For this formulation both the Eosin Y and the Phloxine B are formulated at 0.1% dye content.Eosin Y solution, alcoholicHT110116, HT110132, HT110180, HT1101128Eosin Y solution, aqueousHT110216, HT110232, HT110280, HT1102128Eosin Y solution, alcoholic, with phloxineHT110316, HT110332, HT110380, HT1103128
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