推薦產品
重組細胞
expressed in E. coli (AP401 (k))
形狀
solution
用途
sufficient for 100 reactions
sufficient for 20 reactions
包裝
pkg of 1,000 U (11785826001 [20 μl])
pkg of 5,000 U (11785834001 [100 μl])
製造商／商標名
Roche
參數
42-43 °C optimum reaction temp.
技術
RT-PCR: suitable
RT-qPCR: suitable
輸入
purified RNA
檢測方法
probe-based
儲存溫度
−20°C
一般說明
Expand™逆转录酶是一种RNA指导的DNA聚合酶，经过优化在两步法 RT-PCR检测中可以将cDNA扩增为13.5 kb 片段。该酶是莫洛尼鼠白血病病毒逆转录酶（M-MuLV-RT）的基因工程产品。核糖核酸酶H序列中的A点突变会使核糖核酸酶H活性降低至无法检出的水平。与采用天然M-MuLV-RT相比可以得到明显的改进，包括全长cDNA转录本数量更多且转录本更长
特異性
热灭活：95 °C下处理2分钟
适用于3kb RT-PCR反应的逆转录酶
适用于3kb RT-PCR反应的逆转录酶
應用
Expand™逆转录酶已被用于实时PCR 分析，可以使分离的RNA转化，进入cDNA。它还被用于逆转录（RT）。Expand™逆转录酶也可用于分析 cDNA 的序列并与基因组序列进行比较。 可以获得有关间隔顺序（内含子）和基因组重组的重要信息。
特點和優勢
- 获得更多的全长cDNA转录本和更长的高达13.5kb的转录本
- 没有核糖核酸酶 H活性
- 高效地在二级结构中转录总RNA、mRNA、病毒RNA和富含RNA物质。
- 两步法 RT-PCR检测的理想选择
- 在PCR反应前不需要纯化cDNA
包裝
包含3个组分。
單位定義
一单位逆转录酶的定义为，在+37 °C下，以 poly(A)+ × (dT)15为底物，将10 nmol TMP整合为酸性不可溶产物所需的酶量。
体积活性：50 U/μl
体积活性：50 U/μl
其他說明
仅用于生命科学研究。不可用于诊断。
法律資訊
Expand is a trademark of Roche
僅套裝組件
產品號碼
描述
- Expand Reverse Transcriptase 50 U/μl
- Expand Reverse Transcriptase Buffer 5x concentrated
- DTT Stock Solution 100 mM
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°C）
does not flash
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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相關內容
RT-qPCR 可檢測特定目標，應用於基因表達和病原體檢測。
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
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