11412272001

Transforming Growth Factor-β1, human (hTGF-β1)

recombinant (CHO cells)

• 同義詞: hTGF
• 分類程式碼代碼: 12352207

屬性

• 生物源: human
• 品質等級: 100
• 重組細胞: expressed in CHO cells
• 化驗: >95% (SDS-PAGE)
• 形狀: solution
• 效力: <0.1 ng/mL EC₅₀
• 分子量: 25,000 Da
• 包裝: pkg of 1 mL (1 μg)
• 製造商／商標名: Roche
• 儲存條件: avoid repeated freeze/thaw cycles
• 雜質: <0.1 EU/μgtested (LAL test)
• UniProt登錄號: P01137
• 儲存溫度: −20°C
• 基因資訊: human ... TGFB1(7040)

描述

一般說明

Recombinant, human TGF- β1 is produced in CHO (Chinese Hamster Ovary) cells and purified by standard chromatographic techniques.
Solution in PBS and 1 mg/ml BSA, filtered through 0.2 μm pore size membrane.

特異性

Species specificity: Transforming growth factor-β1 (TGF-β1) is effective on human and mouse cells.
Specific activity/EC₅₀: <0.1 ng/ml, at least the same specific activity (EC<SUB>50) compared to the indicated standard is guaranteed.

應用

Transforming growth factor-β1, human (hTGF-β1) has been used to explore function and underlying mechanism of periostin (PN) during orthodontic periodontal ligament remodeling.

生化／生理作用

Transforming Growth Factor-β1, human (hTGF-β1), is a multifunctional growth factor affecting many functions in a variety of different cells.

Transforming growth factor-β (TGF-β) family comprises of a group of multifunctional cytokines which are involved in controlling apoptosis, growth and differentiation of cells, and play important role during embryo development.

品質

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
(Note: 1 EU corresponds to 0.1 ng)

序列

Chain Length 2 x 112 AA

Two identical polypeptide chains (112 amino acids) connected by disulfide bridges, identical to natural hTGF-β1.

單位定義

EC₅₀ definition: The concentration of hTGF-β1 that is required to mediate half maximal inhibition of DNA synthesis (BrdU incorporation) with Mv1-Lu cells.

準備報告

Working concentration: 0.01 - 10 ng/ml
Recombinant, human TGF-β 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml.
Working solution: Dilute the concentrated TGF-β1 solution (1 μg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%.
Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

其他說明

仅用于生命科学研究。不可用于诊断。

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: does not flash
• 閃點（°C）: does not flash