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Merck
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10775835001

Roche

牛血清白蛋白

98.5% (electrophoresis), Fatty acids (total) ≤0.2 mg/g, Triglycerides (enzym.) free, Immunoglobulins not detectable, microbiological culture: suitable, USA origin

同義詞:

BSA, Bovine Serum Albumin Fraction V, fatty acid free, Bovine albumin, bovine serum

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About This Item

分類程式碼代碼:
12352106

生物源

bovine

品質等級

化驗

98.5% (electrophoresis)

形狀

lyophilized

分子量

Mr 68 kDa

包裝

pkg of 50 g

製造商/商標名

Roche

來源

USA origin

技術

immunofluorescence: suitable
ligand binding assay: suitable

雜質

Immunoglobulins, none detected
Triglycerides, none detected
≤0.2 mg/g fatty acids

UniProt登錄號

運輸包裝

wet ice

儲存溫度

2-8°C

基因資訊

bovine ... ALB(280717)

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一般說明

Highest quality for fatty acid and lipid research.
BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7, it contains 17 intra-chain disulfide bridges and one sulfhydryl group.

應用

Bovine Serum Albumin, Fraction V, fatty acid free is used:
  • in lipid research to study lipid metabolism in biological systems
  • to identify transport mechanisms in living organisms
  • to study ligand binding
  • to investigate ionic binding
  • in the preparation of solutions during isolation of cardiomyocytes
  • as a blocking solution to incubate embryos for immunofluorescence
  • in dual polarisation interferometry (DPI) to study the interaction between membrane active peptides and artificial bilayers

生化/生理作用

The fatty acids that are insoluble in circulating plasma are transported primarily by serum albumin. The human serum albumin and bovine serum albumin share 76% sequence homology. Being the most abundant protein in the circulatory system, it participates in the maintenance of blood pH and carries several endogenous and exogenous compounds, including fatty acids, metal, amino acids, steroids and drugs.

特點和優勢

  • Free from fatty acids according to current quality control procedures (suitable for lipid research)
  • High quality
  • Low content of heavy metal ions
  • Long-term stability

品質

Purity: ≥98.5 % albumin (electrophoresis), 98% protein
Contaminants: <0.2 mg fatty acids/g, triglycerides not detectable (enzymatic), immunoglobulins not detectable (Ouchterlony), ≤5% H2O,≤30 ppm heavy metals

其他說明

仅用于生命科学研究。不可用于诊断。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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存取文件庫

Putnam F
The Plasma Proteins: Structure, Function, and Genetic Control, 5 (2012)
Examination of the Interaction between a Membrane Active Peptide
and Artificial Bilayers by Dual Polarisation Interferometry
Payne JAE, et al.
Bio-protocol, 7(1), 1-12 (2017)
Relief of endoplasmic reticulum stress enhances DNA damage repair and improves development of pre-implantation embryos
Dicks N, et al.
PLoS ONE, 12(11), e0187717-e0187717 (2017)
Tiziana Caputo et al.
Cellular and molecular life sciences : CMLS, 78(1), 227-247 (2020-03-12)
Chronic inflammation that affects primarily metabolic organs, such as white adipose tissue (WAT), is considered as a major cause of human obesity-associated co-morbidities. However, the molecular mechanisms initiating this inflammation in WAT are poorly understood. By combining transcriptomics, ChIP-seq and
Jelena Branovets et al.
American journal of physiology. Heart and circulatory physiology, 305(4), H506-H520 (2013-06-25)
Disruption of the creatine kinase (CK) system in hearts of CK-deficient mice leads to changes in the ultrastructure and regulation of mitochondrial respiration. We expected to see similar changes in creatine-deficient mice, which lack the enzyme guanidinoacetate methyltransferase (GAMT) to

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