1.12018

Albumin fraction V

(from bovine serum) for biochemistry

• 同義詞: Albumin fraction V, Bovine serum albumin, Serum albumin
• CAS號碼: 90604-29-8
• MDL號碼: MFCD00000745
• 分類程式碼代碼: 12352202
• 酶委員會索引編號: 292-322-5
• NACRES: NA.21

屬性

• 生物源: bovine
• 形狀: solid
• 濃度: ≥97% protein (spectralphotometric; calc. on anhydrous substance); ≥98% (Assay of Albumin (Agarose gel electrophoresis))
• 技術: immunoassay: suitable; western blot: suitable
• pH值: 6.8-7.2 (20 °C, 1 g/L in H₂O)
• 溶解度: 1 g/L
• 體積密度: 150 kg/m³
• 儲存溫度: 2-8°C

描述

一般說明

Bovine serum albumin (BSA) belongs to the serum albumin family with three domains and two sub-domains each.[1][2] This globular, α-helical, non-glycosylated protein has 17-disulfide bonds.[1][2]

應用

Albumin fraction V has been used:

• as a component of perfusion buffer for perfusion protocol for rodent organ perfusion [3]
• as a blocking agent in western blot analysis[4]
• as a nitrated protein standard to study the effects of antibodies against 3-nitrotyrosine and to characterize the binding and detection of nitrated proteins in one-sided and sandwich immunoassays[5]

生化／生理作用

Bovine Serum Albumin (BSA) is the chief component of cell culture media[6] and also works as a blocking agent in enzyme-linked immunosorbent assay (ELISA).[7] It is beneficial for embryonic stem cells (hESC) differentiation and assists the transport of drugs, hormones, and fatty acids.[6][1] 

分析報告

Appearance (colour): slightly yellow
Appearance (description): leafs
Assay of protein (spectralphotometric; calc. on anhydrous substance): ≥ 97.0 %
Assay of Albumin (Agarose gel electrophoresis): ≥ 98.0 %
pH-value (1 %; sodium chloride solution c = 0.5 mol/ l): 6.8 - 7.2
K (Potassium): ≤ 0.02 %
Na (Sodium): ≤ 0.5 %
Mg (Magnesium): ≤ 0.01 %
Ca (Calcium): ≤ 0.05 %
Heavy metals (as lead): ≤ 0.001 %
Fats: ≤ 0.2 %
Decomposition products: not detectable
Water (according to Karl Fischer): ≤ 5.00 %
Ash (600 °C): ≤ 1 %
NADH Oxidase (NADH; pH 7.5; 25° C): not detectable
LDH (pyruvate; pH 7.5; 25° C): not detectable

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable